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GrainGenes Reference Report: TAG-102-1273

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Reference
TAG-102-1273
Title
Extending a RFLP-based genetic map of rye using random amplified polymorphic DNA (RAPD) and isozyme markers
Journal
Theoretical and Applied Genetics
Year
2001
Volume
102
Pages
1273-1279
Author
Masojc P
Myskow B
Milczarski P
Abstract
Summary: RFLP-based genetic map of rye, developed previously using a cross of lines DS2xRXL10 (F2 generation), was extended with 69 RAPD and 12 isozyme markers. The actual map contains 282 markers dispersed on all seven chromosomes and spans a distance of 1,140 cM. The efficiency of mapping RAPD markers was close to ten loci per 100-screened arbitrary primers. A strong selection of polymorphic, intensive and reproducible fragments was necessary to reveal individual marker loci that could be assigned to rye chromosomes. Newly mapped markers cover a substantial part of the rye genome and constitute a valuable tool suitable for map saturation, marker-aided selection and phenetic studies. A specific nomenclature for the RAPD loci mapped on individual rye chromosomes, which could be helpful in managing of accumulating data, is proposed
External Databases
http://link.springer-ny.com/link/service/journals/00122/bibs/1102008/11021273.htm
Gene
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Sod-R2 (Secale)
Isa1 (Secale)
Wsp-R2 (Secale)
Ia1 (Secale)
Aat4 (Secale)
Ssp1 (Secale)
Ssp3 (Secale)
alpha-Amy-R1.1 (Secale)
alpha-Amy-R1.2 (Secale)
Per-R5 (Secale)
Dw (Secale)
Map Data
Rye, Ds2 x RxL10, RAPD
Probe
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APR1.5
APR1.2
APR1.6
APR1.3
APR1.7
APR1.1
APR1.4
APR2.5
APR2.10
APR2.8
APR2.7
APR2.11
APR2.2
APR2.3
APR2.6
APR2.9
APR2.12
APR2.4
APR2.1
APR3.5
APR3.6
APR3.7
APR3.1
APR3.2
APR3.3
APR3.4
APR4.9
APR4.7
APR4.11
APR4.3
APR4.4
APR4.5
APR4.10
APR4.2
APR4.1
APR4.8
APR4.6
APR4.12
APR5.3
APR5.4
APR5.7
APR5.1
APR5.9
APR5.6
APR5.8
APR5.5
APR5.2
APR6.8
APR6.5
APR6.2
APR6.1
APR6.4
APR6.7
APR6.3
APR6.6
APR6.9
APR7.11
APR7.1
APR7.2
APR7.12
APR7.13
APR7.6
APR7.4
APR7.8
APR7.9
APR7.7
APR7.5
APR7.10
APR7.3
Keyword
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amplified polymorphic dna
arbitrary primers
chromosome
cross
crosses
distance
dna
fragments
generation
genetic
genetic map
genome
isozyme
isozyme markers
line
linkage map
loci
map
mapping
marker
marker loci
marker-aided selection
nomenclature
p
pcr
plant science
polymorphic
polymorphic dna
primer
random amplified polymorphic dna
rapd
rapd markers
rflp
rye
rye chromosomes
secale cereale
selection
span
tool