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GrainGenes Reference Report: TAG-108-95

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Reference
TAG-108-95
Title
Identification of QTLs associated with Fusarium head blight resistance in Zhedar 2 barley
Journal
Theoretical and Applied Genetics
Year
2003
Volume
108
Pages
95-104
Author
Dahleen LS
[ Show all 7 ]
Abstract
Summary: Fusarium head blight (FHB) in barley and wheat, caused by Fusarium graminearum, is a continual problem worldwide. Primarily, FHB reduces yield and quality, and results in the production of the toxin deoxynivalenol (DON), which can affect food safety. Identification of QTLs for FHB severity, DON level and related traits heading-date (HD) and plant-height (HT) with consistent effects across a set of environments, would provide the basis for marker-assisted selection (MAS) and potentially increase the efficiency of selection for resistance. A segregating population of 75 double-haploid lines, developed from the three-way cross Zhedar 2/ND9712//Foster, was used for genome mapping and FHB severity evaluation. A linkage map of 214 RFLP, SSR and AFLP markers was constructed. Phenotypic data were collected in replicated field trials from five environments in two growing seasons. The data were analyzed using MQTL software to detect quantitative trait locus (QTL) x environment (E) interactions. Because of the presence of QTL x E, the MQM procedure in MAPQTL was applied to identify QTLs in single environments. We identified nine QTLs for FHB severity and five for low DON. Many of the disease-related QTLs identified were coincident with FHB QTLs identified in previous studies. Only two of the QTLs identified in this study were consistent across all five environments, and both were Zhedar 2 specific. Five of the FHB QTLs were associated with HD, and two were associated with HT. Regions that appear to be promising candidates for MAS and further genetic analysis include the two FHB QTLs on chromosome 2H and one on 6H, which were also associated with low DON and later heading-date in multiple environments. This study provides a starting point for manipulating Zhedar 2-derived resistance by MAS in barley to develop cultivars that will show effective resistance under disease pressure
External Databases
Pubmed: 14556050
Map Data
Barley, Zhedar2, FHB QTL
QTL
QFhs.dah-1H.1
[ Show all 21 ]
Trait Study
FHB severity, Dahleen03
DON levels, Dahleen03
Heading date, Dahleen03
Plant height, Dahleen03
Keyword
[ Hide all but 1 of 45 ]
aflp
aflp marker
barley
blight
candidates
chromosome
cross
cultivar
deoxynivalenol
disease
disease resistance
environment
field
field trial
food
fusarium graminearum
fusarium head blight
gene mapping
genetic analysis
genome
graminearum
head blight
head blight resistance
head-blight
heading date
high-resolution
hordeum vulgare
linkage
linkage map
locus
map
mapping
molecular marker
mycotoxins
population
quality
quantitative trait loci
quantitative trait locus
resistance
rflp
rice
selection
specific
ssr
yield