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GrainGenes Reference Report: BLT-73-772

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Reference
BLT-73-772
Title
Identification and antifungal assay of a wheat beta-1,3-glucanase.
Journal
Biotechnology Letters
Year
2009
Volume
73
Pages
772-775
Author
Brueggeman R
[ Show all 16 ]
Abstract
A wheat beta-1,3-glucanase gene (TaGluD) was identified as a fungal defense candidate. Its transcript induction was more than 60-fold higher in a resistant wheat line, Shannong0431, than in a susceptible wheat line, Wenmai6, after infection with Rhizoctonia cerealis. The TaGluD protein was overexpressed as inclusion bodies in Escherichia coli. After refolding and purification, TaGluD with 1 unit of beta-1,3-glucanase showed antifungal activity in vitro against Rhizoctonia solani, R. cerealis, Phytophthora capsici and Alternaria longipes with inhibition rates of 42%, 43%, 32% and 30%, respectively. Thus TaGluD may be useful for enhancing fungal resistance in several crop species.
External Databases
http://dx.doi.org/10.1007/s10529-009-9958-8