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GrainGenes Reference Report: TAG-128-645

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Reference
TAG-128-645
Title
Fine mapping and characterization of Sr21, a temperature-sensitive diploid wheat resistance gene effective against the Puccinia graminis f. sp. tritici Ug99 race group
Journal
Theoretical and Applied Genetics
Year
2015
Volume
128
Pages
645-656
Author
Chen S
[ Show all 7 ]
Abstract
A race of Pucciniagraminis f. sp. tritici, the causal pathogen of stem rust of wheat, known as Ug99, and its variants, are virulent to plants carrying stem rust resistance genes currently deployed in most wheat cultivars worldwide. Therefore, identification, mapping and deployment of effective resistance genes are critical to reduce this threat. Resistance gene Sr21 identified in diploid wheat T. monococcum can be effective against races from the Ug99 race group, but both susceptible and partial resistant reactions have been reported in previous studies. To clarify this conflicting information we screened four monogenic lines with Sr21 and four susceptible controls with 16 Pgt isolates including five isolates of the Ug99 race group under three different temperatures and three different photoperiods. We observed that, temperature influences the interaction between monogenic lines with Sr21 and Ug99 race group isolates, and may be one source of previous inconsistencies. This result indicates that, although Sr21 confers partial resistance against Ug99, its effectiveness can be modulated by environmental conditions and should not be deployed alone. Using two large diploid wheat-mapping populations (total 3,788 F2 plants) we mapped Sr21 approximately 50 cM from the centromere on the long arm of chromosome 2Am within a 0.20 cM interval flanked by sequence-based markers FD527726 and EX594406. The closely linked markers identified in this study will be useful to reduce the T. monococcum segments introgressed into common wheat, accelerate Sr21 deployment in wheat breeding programs, and facilitate the map-based cloning of this gene.
External Databases
http://link.springer.com/article/10.1007%2Fs00122-015-2460-x
PubMed: 25634104
Gene
Sr21 (Triticum)
Locus
Sr21
Probe
FD527726
EX594406
Summarized in
ReferenceMAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr21 MAS Wheat. Marker Assisted Selection in Wheat.