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GrainGenes Reference Report: TAG-128-605

Reference
TAG-128-605
Title
Mapping resistance to the Ug99 race group of the stem rust pathogen in a spring wheat landrace
Journal
Theoretical and Applied Genetics
Year
2015
Volume
128
Pages
605-612
Author
Babiker EM
[ Show all 11 ]
Abstract
Wheat landrace PI 374670 has seedling and field resistance to stem rust caused by Puccinia graminis f. sp tritici Eriks. & E. Henn (Pgt) race TTKSK. To elucidate the inheritance of resistance, 216 BC1F2 families, 192 double haploid (DH) lines, and 185 recombinant inbred lines (RILs) were developed by crossing PI 374670 and the susceptible line LMPG-6. The parents and progeny were evaluated for seedling resistance to Pgt races TTKSK, MCCFC, and TPMKC. The DH lines were tested in field stem rust nurseries in Kenya and Ethiopia. The DH lines were genotyped with the 90K wheat iSelect SNP genotyping platform. Goodness-of-fit tests indicated that a single dominant gene in PI 374670 conditioned seedling resistance to the three Pgt races. The seedling resistance locus mapped to the long arm of chromosome 7A and this result was verified in the RIL population screened with the flanking SNP markers using KASP assays. In the same region, a major QTL for field resistance was detected in a 7.7 cM interval and explained 34–54 and 29–36 % of the variation in Kenya and Ethiopia, respectively. Results from tests with specific Pgt races and the csIH81 marker showed that the resistance was not due to Sr22. Thus, a new stem rust resistance gene or allele, either closely linked or allelic to Sr15, is responsible for the seedling and field resistance of PI 374670 to Ug99.
External Databases
http://link.springer.com/article/10.1007%2Fs00122-015-2456-6
PubMed: 25599859
Gene
Sr22 (Triticum)
Sr15 (Triticum)
Locus
IWB48036
IWA4434
IWB46162
Probe
IWB28967
IWAB8036
IWA4434
IWB46162
QTL
QSr.abr-7AL
QSr.abr-2D
Summarized in
ReferenceMAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. QSr.abr-7AL MAS Wheat. Marker Assisted Selection in Wheat.