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GrainGenes Reference Report: TPJ-89-853

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Reference
TPJ-89-853
Title
Towards a whole-genome sequence for rye (Secale cereale L.).
Journal
The Plant Journal
Year
2017
Volume
89
Pages
853-869
Author
Bauer E
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Abstract
We report on a whole-genome draft sequence of rye (Secale cereale L.). Rye is a diploid Triticeae species closely related to wheat and barley, and an important crop for food and feed in Central and Eastern Europe. Through whole-genome shotgun sequencing of the 7.9-Gbp genome of the winter rye inbred line Lo7 we obtained a de novo assembly represented by 1.29 million scaffolds covering a total length of 2.8Gbp. Our reference sequence represents nearly the entire low-copy portion of the rye genome. This genome assembly was used to predict 27784 rye gene models based on homology to sequenced grass genomes. Through resequencing of 10 rye inbred lines and one accession of the wild relative S.vavilovii, we discovered more than 90 million single nucleotide variants and short insertions/deletions in the rye genome. From these variants, we developed the high-density Rye600k genotyping array with 600843 markers, which enabled anchoring the sequence contigs along a high-density genetic map and establishing a synteny-based virtual gene order. Genotyping data were used to characterize the diversity of rye breeding pools and genetic resources, and to obtain a genome-wide map of selection signals differentiating the divergent gene pools. This rye whole-genome sequence closes a gap in Triticeae genome research, and will be highly valuable for comparative genomics, functional studies and genome-based breeding in rye.
External Databases
PubMed: 27888547
Map Data
Rye, Lo7 x Lo225 600K
Probe
AX-99222660
[ Show all 85545 ]
Sequence
AX-99222660
[ Show all 85545 ]
Keyword
de novo genome assembly
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