GrainGenes Reference Report: PBR-129-53
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| | Identification and molecular tagging of a stripe rust resistance gene in wheat line P81 |
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| | Stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most devastating diseases in common wheat (Triticum aestivum L.). With the objective of identifying and tagging a new gene for resistance to stripe rust in wheat line P81, F1, F2 and F2:3 populations from the cross 'Chuanmai 28'/P81 were inoculated with Chinese PST race CYR32 in greenhouse and field trials. P81 carried a single dominant gene for resistance (designated YrP81) to CYR32. Tests of allelism showed that YrP81 was different from Yr5, Yr10, Yr15 and Yr26. Simple sequence repeat (SSR) and resistance gene-analogue polymorphism (RGAP) between the parents were used for genotyping the F2 populations. YrP81 was closely linked to four SSR loci on chromosome 2BS with genetic distances of 18.3 cM (Xwmc25), 1.8 cM (Xgwm429), 4.1 cM (Xwmc770) and 5.3 cM (Xgwm148). Two RGAP markers RGA1 (NLRR/XLRR) and RGA2 (Pto kin4/NLRR-INV2) were also closely linked to YrP81 with genetic distances of 4.7 and 6.3 cM, respectively. The linkage map of YrP81 and molecular markers was established in the order Xwmc25-RGA2-RGA1-Xgwm429-YrP81-Xwmc770-Xgwm148. Pedigree analysis, response patterns with Chinese PST races and associations with markers suggested that YrP81 is a novel stripe rust resistance gene. The PCR-based microsatellite and RGAP markers identified here could be applied in selection of YrP81 in wheat breeding. |
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