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GrainGenes Reference Report: BMG-18-567

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Reference
BMG-18-567
Title
Development of a high-density linkage map and mapping of the three-pistil gene (Pis1) in wheat using GBS markers
Journal
BMC Genomics
Year
2017
Volume
18
Pages
567-574
Author
Yang Z
[ Show all 6 ]
Abstract
Background: The wheat mutant line three-pistil (TP) exhibits three pistils per floret. As TP normally has two or three seeds in each of the florets on the same spike, there is the possibility of increasing the number of grains per spike. Therefore, TP is a highly valuable mutant for breeding and for the study of floral development in wheat. To map the three-pistil gene (Pis1), genotyping-by-sequencing single-nucleotide polymorphism (GBS-SNP) data from an F2 mapping population (CM28 x CM28TP) was used to construct a genetic map that is of significant value. Results: In the present study, a high-density genetic map of wheat containing 2917 GBS-SNP markers was constructed. Twenty-one linkage groups were resolved, with a total length of 2371.40 cM. The individual chromosomes range from 2.64 cM to 454.55 cM with an average marker density of 0.81 cM. The Pis1 gene was mapped using this high-resolution map, and two flanking SNP markers tightly linked to the gene, M70 and M71, were identified. The Pis1 is 3.00 cM from M70 and 1.10 cM from M71. In bread wheat genome, M70 and M71 were found to delimit a physical distance of 3.40 Mb, which encompasses 127 protein-coding genes. To validate the GBS-generated genotypic data and to eliminate missing marker data in the Pis1 region, five Kompetitive Allele-Specific PCR (KASP) assays were designed from corresponding GBS sequences, which harbor SNPs that surround Pis1. Three KASP-SNP markers, KM70, KM71, and KM75, were remapped to the Pis1 gene region. Conclusions : This work not only lays the foundation for the map-based cloning of Pis1 but can also serve as a valuable tool for studying marker-trait association of important traits and marker-assisted breeding in wheat.
External Databases
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-3960-7
Gene
Pis1 (Triticum)
[ Show all 90 ]
Gene Class
Multi-gynoecium
Germplasm
Chuanmai 28
CM28TP
Locus
M70
[ Show all 124 ]
Map Data
Wheat, CM28xCM28TP, Pis1 QTL
Probe
K2DL_CSv1_136805221
K2DL_CSv1_140258883
QTL
QPis1.CM28TP-2D
Keyword
Kompetitive allele-specific PCR (KASP)
Three-pistil mutation
Wheat Genotyping-by-sequencing (GBS)

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