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GrainGenes Reference Report: PBJ-15-390

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Reference
PBJ-15-390
Title
Characterization of a Wheat Breeders' Array suitable for high-throughput SNP genotyping of global accessions of hexaploid bread wheat (Triticum aestivum)
Journal
Plant Biotechnology Journal
Year
2017
Volume
15
Pages
390-401
Author
Allen AM
[ Show all 21 ]
Abstract
Targeted selection and inbreeding have resulted in a lack of genetic diversity in elite hexaploid bread wheat accessions. Reduced diversity can be a limiting factor in the breeding of high yielding varieties and crucially can mean reduced resilience in the face of changing climate and resource pressures. Recent technological advances have enabled the development of molecular markers for use in the assessment and utilization of genetic diversity in hexaploid wheat. Starting with a large collection of 819 571 previously characterized wheat markers, here we describe the identification of 35 143 single nucleotide polymorphism-based markers, which are highly suited to the genotyping of elite hexaploid wheat accessions. To assess their suitability, the markers have been validated using a commercial high-density Affymetrix Axiom(R) genotyping array (the Wheat Breeders' Array), in a high-throughput 384 microplate configuration, to characterize a diverse global collection of wheat accessions including landraces and elite lines derived from commercial breeding communities. We demonstrate that the Wheat Breeders' Array is also suitable for generating high-density genetic maps of previously uncharacterized populations and for characterizing novel genetic diversity produced by mutagenesis. To facilitate the use of the array by the wheat community, the markers, the associated sequence and the genotype information have been made available through the interactive web site 'CerealsDB'.
External Databases
https://doi.org/10.1111/pbi.12635
Locus
AX-95070074
[ Show all 35143 ]
Map Data
Wheat, Axiom 2018, AxC
[ Show all 6 ]
Probe
AX-95070074
[ Show all 35143 ]
Sequence
AX-95070074
[ Show all 35143 ]

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