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GrainGenes Reference Report: CRL-53-247

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Reference
CRL-53-247
Title
Primary structure of a C hordein gene from barley
Journal
Carlsberg Research Communications
Year
1988
Volume
53
Pages
247-258
Author
Entwistle J
Type
Article
Language
English
Abstract
The nucleotide sequence of a 2065 base pair HindIII fragment, containing a gene (.lambda.hor1-14) belonging to the Hor1 locus in barley, has been determined. The fragment consists of 1044 bp of coding region interrupted by an amber codon at base 481, a 5' non- coding region of 428 bp and a 3' non-coding region with 593 bp. The deduced amino acid sequence of the mature protein (327 amino acids) is characterized by an octapeptide motif PQQPFPQQ which is repeated thoughout the peptide chain between a unique 12 amino acid long NH2-terminal and an equally unique 10 amino acid long COOH-terminal end. The proline + glutamine content is 62% and the next three most abundant amino acids are leucine (9%), phenylalanine (8%) and isoleucine (3%). In the 5' non-coding region there is a TATA box at -98 bp from the start methionine. The 3' non-coding region has a polyadenylation signal 76 bp downstream from the TAA stop codon. The deduced amino acid sequences of the NH2- and COOH-terminals of .lambda.hor1-14 are very similar but not identical to those known from the Edman degradation and carboxypeptidase Y analysis of C- hordein polypeptides. The 3' coding and non-coding region of .lambda.hor1-14 is closely similar but different in detail to the known C-hordein cDNA clones. One polyadenylation signal is found in .lambda.hor1-14 whereas two are present in each of the three known C-hordein cDNAs. These differences and the amber codon interrupting the open reading frame indicate that this gene is silent.
Gene
Hor1 (Hordeum)
Probe
pc P387
lambda-hor1-14
Sequence
M36941