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GrainGenes Reference Report: CRS-33-453

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Reference
CRS-33-453
Title
RFLP analysis of genomic regions associated with resistance to pre-harvest sprouting in wheat
Journal
Crop Science
Year
1993
Volume
33
Pages
453-459
Author
Anderson JA
Sorrells ME
Tanksley SD
Type
Article
Language
English
Abstract
Resistance to pre-harvest sprouting (PHS) is difficult to incorporate into new varieties because heritability is low and selection is limited to one generation per year. Our objective was to identify genomic regions containing quantitative trait loci associated with resistance to PHS in two recombinant inbred (RI) populations of white wheat (Triticum aestivum L. em. Thell.) using restriction fragment length polymorphism (RFLP) markers. One population consisted of 78 RI lines from the cross of NY6432-18 (NY18) x 'Clark's Cream' (CC). The second population consisted of 138 RI lines from the cross between sib lines NY18 and NY6432-10 (NY10). The NY18/CC and NY18/10 populations were evaluated for PHS in 6 and 7 environments, respectively, by examining physiologically mature spikes under simulated rainfall. The three parental lines were surveyed for polymorphism with 195 low-copy RFLP clones using four restriction enzymes. Individual RI lines from the NY18/CC and NY18/NY10 populations were probed with 37 and 27 polymorphic clones, respectively. Eight regions of the genome (four from each population) were significantly associated with resistance to PHS. Using multiple regression, specific sets of markers and their interactions accounted for 44 and 51% of the genetic variance for PHS in the NY18/CC and NY18/NY10 populations, respectively. These markers could find utility in breeding programs as indirect selection criteria for improvement of PHS resistance.
Locus
Sprouting 1
Map Data
Wheat sprouting markers
Trait Study
Sprouting, Anderson93
2 Point Data
Sprouting 1/Xbcd1434