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GrainGenes Reference Report: EJB-158-57

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Reference
EJB-158-57
Title
Structure and expression of ubiquitin genes in higher plants
Journal
European Journal of Biochemistry
Year
1986
Volume
158
Pages
57-62
Author
Gausing K
Barkardottir R
Type
Article
Language
English
Abstract
cDNA clones encoding ubiquitin were isolated from a barley leaf cDNA library using a mammalian ubiquitin cDNA clone as probe. The nucleotide sequence of one of the clones codes for 2.2 perfect repeats of the 76-amino-acid-long ubiquitin protein with an extra lysine residue at the C-terminus. The barley ubiquitin amino acid sequence differs from the animal sequence at three positions and from the yeast sequence at two positions. The ubiquitin poly(precursor) are coded by a multigene family with 8-10 genes that produce four or five different size messengers between 700 and 2000 nucleotides in length. The large poly(A)-rich RNAs are constitutively expressed in vegetative tissues whereas the 700- nucleotide messenger(s) were only detected in tissues containing dividing cells.
Probe
pKG3730
Keyword
Amino-acid sequence
Animal
Barley
Yeast