Query (optional)   in Class  

GrainGenes Reference Report: GEN-149-2007

[ Printable Version ]  [ Submit comment/correction ]

Reference
GEN-149-2007
Title
A microsatellite map of wheat
Journal
Genetics
Year
1998
Volume
149
Pages
2007-2023
Author
Roder MS
[ Show all 9 ]
Abstract
Hexaploid bread wheat (Triticum aestivum L. em. Thell) is one of the world's most important crop plants and displays a very low level of intraspecific polymorphism. We report the development of highly polymorphic microsatellite markers using procedures optimized for the large wheat genome. The isolation of microsatellite-containing clones from hypomethylated regions of the wheat genome increased the proportion of useful markers almost twofold. The majority (80%) of primer sets developed are genome-specific and detect only a single locus in one of the three genomes of bread wheat (A, B, or D). Only 20% of the markers detect more than one locus. A total of 279 loci amplified by 230 primer sets were placed onto a genetic framework map composed of RFLPs previously mapped in the reference population of the International Triticeae Mapping Initiative (ITMI) Opata 85 x W7984. Sixty-five microsatellites were mapped at a LOD >2.5, and 214 microsatellites were assigned to the most likely intervals. Ninety-three loci were mapped to the A genome, 115 to the B genome, and 71 to the D genome. The markers are randomly distributed along the linkage map, with clustering in several centromeric regions.
External Databases
Pubmed: 9691054
Locus
Xgwm99-1A
[ Show all 278 ]
Map Data
Wheat, Synthetic x Opata, SSR
Polymorphism
WMS2
[ Show all 230 ]
Probe
WMS99
[ Show all 229 ]
Image
Ta-Synthetic/Opata-SSR group 1 maps
[ Show all 7 ]