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GrainGenes Reference Report: GNM-34-362

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Reference
GNM-34-362
Title
A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD).
Journal
Genome
Year
1991
Volume
34
Pages
362-374
Author
Gill KS
Lubbers EL
Gill BS
Raupp WJ
Cox TS
Type
Article
Language
English
Abstract
One hundred and seventy-eight loci have been mapped in Triticum tauschii (Coss.) Schmal. (2n = 14, DD) and Triticum aestivum L. em. Thell. (2n = 42, AABBDD). Thirty-five loci were mapped by aneuploid analysis in T. aestivum. One hundred and fifty-two loci, including 143 restriction fragment length polymorphisms (RFLPs), 8 proteins, and 1 leaf rust resistance gene, were mapped in an F2 population (60 plants) of T. tauschii. One hundred and twenty-seven loci were placed in linkage groups belonging to seven D-genome chromosomes of T. tauschii. The source of the probes was a PstI genomic library of T. tauschii, which gave 13% single-low copy clones. Four restriction endonucleases (DraI, EcoRI, EcoRV, HindIII) gave 75% polymorphism between the two parents. Nineteen clones detected multiloci ranging from two to nine in number. Deletions-insertions and point mutations were equally important for generating RFLPs. A hypervariable sequence was identified, which may have potential use in varietal fingerprinting. One marker was found to be linked to a rust-resistance gene. The map will be useful for determining genetic relationships in the Triticeae and for tagging genes of economic importance.
Locus
XksuD49-1D
[ Show all 69 ]
Map Data
T.tauschii
Probe
pTtksuG48
[ Show all 26 ]
2 Point Data
Lr21/Gli-D1#2
Keyword
Aegilops squarrosa
[ Show all 20 ]
Agricola Code
IND91037309
Gene Catalogue Number
309
Cited in
ReferenceMcIntosh RA et al. (1995) Catalogue of gene symbols for wheat. Proceedings of the 8th International Wheat Genetics Symposium 1333-1500.