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GrainGenes Reference Report: GNM-34-362

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Reference
GNM-34-362
Title
A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD).
Journal
Genome
Year
1991
Volume
34
Pages
362-374
Author
Gill KS
Lubbers EL
Gill BS
Raupp WJ
Cox TS
Type
Article
Language
English
Abstract
One hundred and seventy-eight loci have been mapped in Triticum tauschii (Coss.) Schmal. (2n = 14, DD) and Triticum aestivum L. em. Thell. (2n = 42, AABBDD). Thirty-five loci were mapped by aneuploid analysis in T. aestivum. One hundred and fifty-two loci, including 143 restriction fragment length polymorphisms (RFLPs), 8 proteins, and 1 leaf rust resistance gene, were mapped in an F2 population (60 plants) of T. tauschii. One hundred and twenty-seven loci were placed in linkage groups belonging to seven D-genome chromosomes of T. tauschii. The source of the probes was a PstI genomic library of T. tauschii, which gave 13% single-low copy clones. Four restriction endonucleases (DraI, EcoRI, EcoRV, HindIII) gave 75% polymorphism between the two parents. Nineteen clones detected multiloci ranging from two to nine in number. Deletions-insertions and point mutations were equally important for generating RFLPs. A hypervariable sequence was identified, which may have potential use in varietal fingerprinting. One marker was found to be linked to a rust-resistance gene. The map will be useful for determining genetic relationships in the Triticeae and for tagging genes of economic importance.
Locus
XksuD49-1D
[ Show all 69 ]
Map Data
T.tauschii
Probe
pTtksuG48
[ Show all 26 ]
2 Point Data
Lr21/Gli-D1#2
Keyword
[ Hide all but 1 of 20 ]
Aegilops squarrosa
chromosome maps
deletions
gene location
gene mapping
genetic markers
genetic resistance
genomes
inversion
leaf rust.
linkage
linkage groups
loci
multiloci
mutations
restriction fragment length polymorphism
restriction mapping
rust diseases
triticum
triticum aestivum
Agricola Code
IND91037309
Gene Catalogue Number
309
Cited in
ReferenceMcIntosh RA et al. (1995) Catalogue of gene symbols for wheat. Proceedings of the 8th International Wheat Genetics Symposium 1333-1500.

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