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GrainGenes Reference Report: GNM-36-517

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Reference
GNM-36-517
Title
Physical mapping of four sites of 5S rDNA sequences and one site of the alpha amylase-2 gene in barley Hordeum-vulgare.
Journal
Genome
Year
1993
Volume
36
Pages
517-523
Author
Leitch IJ
Heslop-Harrison JS
Type
Article
Abstract
The 5S rDNA sequences have been mapped on four pairs of barley (Hordeum vulgare L.) chromosomes using in situ hybridization and barley monotelotrisomic lines. The 5S rDNA sequences are located, genetically and physically, on the short arm of chromosome 1 (7I) and the long arms of chromosomes 2 (2I) and 3 (3I). The 5S rDNA sequence is also located on the physically long arm of chromosome 4 (4I). Only one site on locations of the 5S rDNA sequences make them useful as molecular marker to identify each barley chromosome. The physical position of the low-copy alpha-amylase-2 gene was determined using in situ hybridization; the location of this gene on the long arm of chromosome 1 (7I) was confirmed by reprobing the same preparation with the 5S rDNA probe. The results show that there is a discrepancy between the physical and genetic position of the alpha-amylase-2 gene.
Gene
Rrn5S (Hordeum)
Map Data
Barley, BxGP
Barley, CxHs
Gene Catalogue Number
9439
Cited in
ReferenceMcIntosh RA et al. (1994) Catalogue of gene symbols for wheat, 1994 supplement Annual Wheat Newsletter.