Query (optional)   in Class  

GrainGenes Reference Report: GNM-42-964

[ Printable Version ]  [ Submit comment/correction ]

Reference
GNM-42-964
Title
Development of simple sequence repeat markers in rye (Secale cereale L.)
Journal
Genome
Year
1999
Volume
42
Pages
964-972
Author
Saal B
Wricke G
Abstract
Summary: Simple sequence repeats (SSRs), also referred to as microsatellites, represent a PCR-based marker system that has been described in mammalian and plant genomes in recent years. In self-pollinating crop plants they have been shown to be superior to other DNA markers with respect to their level of polymorphism. The technical advantages compared with RFLP markers should also facilitate marker analysis in outcrossing crops like rye. In order to determine the usefulness of SSR markers in rye genetics and breeding, several genomic libraries were screened for (CT/GA)n and (GT/CA)n dinucleotide repeats. It was estimated that these motifs occur at a frequency of one per 268-519 kb. Seventy four out of 182 positive clones were sequenced, and the majority (56.8%) revealed perfect repeats, predominantly of the type (GT/CA)n(61.9%). Fifty seven primer pairs were designed and 27 (47.4%) resulted in specific SSR markers, of which 20 were genetically mapped or assigned to chromosomes or chromosome arms, respectively. The level of polymorphism of four SSR and three RFLP markers was assessed in two open-pollinated rye cultivars. On average, the SSR markers showed larger values of expected heterozygosity (0.62 vs. 0.43) and allele number (5.9 vs. 3.4) than RFLP markers in both cultivars
External Databases
Pubmed: 10584314
Probe
SCM9
[ Show all 27 ]
Keyword
alleles
[ Show all 24 ]