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GrainGenes Reference Report: JCS-34-285

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Reference
JCS-34-285
Title
Relationships between barley hordeins and malting quality in a mutant of cv. triumph I. Genotype by environment interaction of hordein content
Journal
Journal of Cereal Science
Year
2001
Volume
34
Pages
285-294
Author
Molina-Cano JL
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Abstract
Summary: The genetic (G), environmental (E) and G x E effects on total grain protein and hordein fractions, have been studied in a mutant (TL43) and its parent cultivar, the malting barley Triumph, in various seasons at Dundee (E Scotland) and Lleida (NE Spain). The grain protein content of TL43 was consistently about 2% higher than that of Triumph across environments. Differences in hordein composition were shown by SDS-PAGE electrophoresis. TL-43 had three B-hordein bands not present in Triumph which had a further three bands of the same hordein group not present in TL43. TL43 showed higher B-hordein content than Triumph in Scotland but lower in Spain, whereas the mutant showed consistently higher C- and D-hordein content than Triumph across environments, i.e. there was crossover G x E interaction for B-hordein and non-crossover for C- and D-hordein content. There were also differences in grain ultra-structure between the two lines, as TL43 showed a more dense protein matrix than Triumph, together with thinner pericarp, testa and aleurone layers. It was concluded that the mutation in TL43 had a significant effect on storage protein composition
Keyword
aleurone
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