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GrainGenes Reference Report: MBR-8-187

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Reference
MBR-8-187
Title
Resistance QTL confirmed through development of QTL-NILs for barley leaf rust resistance
Journal
Molecular Breeding
Year
2001
Volume
8
Pages
187-195
Author
van Berloo R
Aalbers H
Werkman A
Niks RE
Abstract
Summary: Near-isogenic lines (NILs) differing with regard to disease QTLs provide valuable material for a more detailed study into the genetic basis of quantitative resistance. Previously obtained information on QTLs that show an effect on leaf rust (Puccinia hordei) in barley was used in a marker-assisted backcross programme. The genome origin in backcross plants was controlled through AFLP marker analysis and graphical genotyping. Plants obtained after the third generation of backcrossing sufficiently resembled the recurrent parent. For one QTL, BC3S1 plants were evaluated in a disease test and genotyped. NILs containing the desired QTL in homozygous condition in a recipient background were finally obtained. A disease test and verification of the marker genotype confirmed the identity of the NILs. Simultaneous with the backcross programme a simulation study on efficiency of marker-assisted backcrossing was performed
Keyword
aflp
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