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GrainGenes Reference Report: MPM-7-657

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Reference
MPM-7-657
Title
High resolution mapping of the Hor1/Mla/Hor2 region on chromosome 5S in barley.
Journal
Molecular Plant Microbe Interactions
Year
1994
Volume
7
Pages
657-666
Author
DeScenzo RA
Wise RP
Mahadevappa M
Type
Article
Abstract
A high resolution mapping population consisting of 270 individual lines, each representing an independent recombination event between the Hor1 and Hor2 loci, was used to construct a high density RFLP map of the Hor1/Mla/Hor2 region. To identify informative markers for screening the recombinant population, the Franger (containing Mla6 and Mla14) and Rupee (containing Mla13) parental lines were screened for RFLPs using 12 restriction endonucleases and 35 cDNA and genomic clones, that had been shown to map to barley chromosome 5 or wheat group 1. As a result, 61 probe/enzyme combinations were chosen to screen the recombinant population. Analysis of the RFLP mapping data indicated 9 of the 21 polymorphic probes mapped within the Hor1/Hor2 interval. Map distance between Hor1 and Hor2 was calculated to be approximately 8.1 cM. Nine of the remaining probes mapped outside of the Hor1/Hor2 interval and proximal to Hor1. The remaining three probes mapped to separate linkage groups. Sixteen of the recombinant lines had regions of DNA which were heterozygous at one or more RFLP loci within the Hor1/Hor2 interval. Data from the high resolution RFLP map of the Hor1/Hor2 region was integrated with previous recombination data which positioned the Mla6, Mla13, and Mla14, resistance alleles, in relation to Hor1 and Hor2. Five of the probes hybridized to multiple sites. BCD249, a cDNA-derived barley clone, hybridized to two sequences 0.3 cM apart, indicating a duplication event within the Hor1/Hor2 interval. One of these sites, Xbcd249.2, was positioned within 1.6 cM from the Mla alleles.
Map Data
Barley, Wise
Keyword
barley
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Image
DeScenzo, 1994 Fig. 2

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