Query (optional)   in Class  

GrainGenes Reference Report: PBR-118-275

[Submit comment/correction]

Reference
PBR-118-275
Title
A polymorphic microsatellite in the 3 ' end of 'waxy' genes of wheat, Triticum aestivum.
Journal
Plant Breeding
Year
1999
Volume
118
Pages
275-277
Author
Shariflou MR
Sharp PJ
Abstract
Summary: Potential polymorphism of an (AT)n microsatellite at the 3' end of waxy genes in bread wheat was examined. Primers were designed from a published cDNA sequence of a wheat waxy gene. Polymerase chain reaction (PCR) amplification of genomic DNA from 135 mainly Australian cultivars revealed eight alleles on chromosomes 7A. This polymorphic microsatellite is a potential codominant marker for the Wx-Al locus in breeding programmes. A distinguishable fragment was also amplified from chromosome 7D. This fragment was absent where a plant was null for the waxy gene on chromosome 7D, being a dominant marker for the Wx-Dl locus. The primers were also useful for amplifying genomic DNA from barley, rye and triticale and can be used to detect potential polymorphism in these species
External Databases
https://doi.org/10.1046/j.1439-0523.1999.118003275.x
Allele
Wx-D1b (Triticum)
Gene
Wx-A1 (Triticum)
Gene Class
Starch synthase
Waxy proteins
Keyword
alleles
[ Show all 13 ]

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.