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GrainGenes Reference Report: CRS-61-490

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Reference
CRS-61-490
Title
Development of KASP markers for wheat greenbug resistance gene Gb5
Journal
Crop Science
Year
2021
Volume
61
Pages
490-499
Author
Xu X
[ Show all 7 ]
Abstract
Greenbug [Schizaphis graminum (Rondani)] is a destructive pest of wheat (Triticum aestivum L.) worldwide. Incorporation of wheat greenbug resistance genes in wheat-breeding pipelines is critical to improve wheat production. The greenbug resistance gene Gb5, originally identified in Triticum speltoides chromosome 7S and transferred to wheat chromosome 7A, confers resistance to several economically important greenbug biotypes. The T. speltoides chromosome segment carrying Gb5 was previously shortened by means of homoeologous recombination induced by the ph1b gene, which minimized linkage drag and made Gb5 a useful gene for improvement of greenbug resistance in wheat. This study characterized the shortened T. speltoides chromosome segment in PI 603919 and developed kompetitive allele-specific polymerase chain reaction (KASP) markers to facilitate its introgression in wheat breeding. A set of 21 simple sequence repeat (SSR) markers specific to wheat 7AL chromosome were developed to determine the length and location of the T. speltoides chromosome segment. The T. speltoides segment in PI 603919 was estimated to be about 79.5–87.8 Mb in the Chinese Spring reference sequence IWGSC RefSeq v.1.0. A recombinant inbred line (RIL) population derived from a cross between CI 17884, which carries the original wheat-T. speltoides 7A-7S translocation chromosome segment, and Bainong 418 was genotyped using the genotyping-by-sequencing (GBS) approach and evaluated for responses to greenbug biotype E. Three GBS-derived single nucleotide polymorphisms (SNPs) on the shortened translocation segment were converted to KASP markers. These KASP markers were evaluated for efficacy to select Gb5 in wheat breeding.
External Databases
https://doi.org/10.1002/csc2.20339
Gene
Gb5 (Triticum)
Locus
Stars760
[ Show all 21 ]
Probe
[ Hide all but 1 of 24 ]
KASP-Gb5-1
KASP-Gb5-2
KASP-Gb5-3
Stars760
Stars762
Stars764
Stars765
Stars767
Stars769
Stars772
Stars773
Stars774
Stars775
Stars800
Stars803
Stars804
Stars805
Stars806
Stars808
Stars809
Stars811
Stars812
Stars813
Stars814

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