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GrainGenes Reference Report: TAG-109-725

Reference
TAG-109-725
Title
Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags
Journal
Theoretical and Applied Genetics
Year
2004
Volume
109
Pages
725-732
Author
Khlestkina EK
[ Show all 7 ]
Abstract
The genetic map of rye contains predominantly restriction fragment length polymorphism (RFLP) markers but also a limited number of microsatellite markers, which are known to be more reliable and easier to apply. We report here the saturation of the genomic map of rye with additional microsatellite-derived markers that we obtained from the rye expressed sequence tag (EST) databases and the Gatersleben collection of wheat microsatellite markers (WMS). A total of 99 loci (39 EST and 60 WMS) were mapped into the RFLP frameworks of four rye mapping populations consisting of 139, 64, 58 and 60 RFLPs, respectively. For another ten WMS loci, which amplified PCR products not polymorphic in any of the mapping populations, chromosome and chromosome arm locations were determined using wheat-rye addition lines. Chromosomes 1R, 2R, 3R, 4R, 5R, 6R and 7R were enriched with 9, 19, 9, 13, 27, 16 and 16 microsatellite loci, respectively. The microsatellite loci mapped were evenly distributed along the chromosomes, which is important for the further application of these markers for gene mapping or diversity studies in rye. Forty-four of the WMS loci mapped in rye were found to be homoeologous to those mapped in bread wheat (Triticum aestivum L.)
Germplasm
N2 (rye)
N6 (rye)
N7 (rye)
Locus
Mdh
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Map Data
Rye, P87 x P105, 2004
Rye, N6 x N2, 2004
Rye, N7 x N2, 2004
Rye, N7 x N6, 2004
Probe
PSR567
[ Show all 81 ]
Keyword
[ Hide all but 1 of 12 ]
chromosome mapping
chromosomes
expressed sequence tags
genetic markers
loci
microsatellite repeats
restriction fragment length polymorphism
rye
secale cereale
triticum aestivum
wheat
wheat microsatellite markers
Image
1R_SSR_2004
[ Show all 7 ]