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GrainGenes Reference Report: TAG-109-725

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Reference
TAG-109-725
Title
Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags
Journal
Theoretical and Applied Genetics
Year
2004
Volume
109
Pages
725-732
Author
Khlestkina EK
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Abstract
The genetic map of rye contains predominantly restriction fragment length polymorphism (RFLP) markers but also a limited number of microsatellite markers, which are known to be more reliable and easier to apply. We report here the saturation of the genomic map of rye with additional microsatellite-derived markers that we obtained from the rye expressed sequence tag (EST) databases and the Gatersleben collection of wheat microsatellite markers (WMS). A total of 99 loci (39 EST and 60 WMS) were mapped into the RFLP frameworks of four rye mapping populations consisting of 139, 64, 58 and 60 RFLPs, respectively. For another ten WMS loci, which amplified PCR products not polymorphic in any of the mapping populations, chromosome and chromosome arm locations were determined using wheat-rye addition lines. Chromosomes 1R, 2R, 3R, 4R, 5R, 6R and 7R were enriched with 9, 19, 9, 13, 27, 16 and 16 microsatellite loci, respectively. The microsatellite loci mapped were evenly distributed along the chromosomes, which is important for the further application of these markers for gene mapping or diversity studies in rye. Forty-four of the WMS loci mapped in rye were found to be homoeologous to those mapped in bread wheat (Triticum aestivum L.)
Germplasm
N2 (rye)
N6 (rye)
N7 (rye)
Locus
Mdh
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Map Data
Rye, P87 x P105, 2004
Rye, N6 x N2, 2004
Rye, N7 x N2, 2004
Rye, N7 x N6, 2004
Probe
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PSR567
PSR330
WMS752
WMS1266
WMS781
PSR547
PSR59
PSR604
PSR945
PSR634
PSR921
PSB37
PSR303
WMS877
WMS676
REMS1135
WMS1166
WMS861
WMS1300
WMS912
REMS1251
WMS1048
REMS1238
REMS1208
REMS1194
REMS1132
WMS991
REMS1138
WMS1296
WMS1134
REMS1154
WMS1255
WMS313
REMS1167
WMS996
WMS1011
WMS1122
REMS1266
REMS1186
REMS1237
REMS1250
WMS984
WMS1081
REMS1187
REMS1234
WMS1223
REMS1303
REMS1130
REMS1230
REMS1254
WMS779
REMS1323
REMS1261
REMS1160
WMS1165
REMS1174
REMS1218
REMS1247
WMS732
WMS1103
REMS1152
WMS1302
REMS1162
REMS1188
REMS1281
REMS1253
WMS959
WMS791
WMS911
WMS1091
WMS941
WMS1284
WMS1205
WMS1226
REMS1280
REMS1203
WMS1059
REMS1264
REMS1205
REMS1259
REMS1197
Keyword
chromosome mapping
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Image
1R_SSR_2004
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