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GrainGenes Reference Report: TAG-83-250

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Reference
TAG-83-250
Title
Construction of an RFLP map of barley
Journal
Theoretical and Applied Genetics
Year
1991
Volume
83
Pages
250-256
Author
Graner A
[ Show all 8 ]
Language
English
Abstract
In order to construct an RFLP map of barley [Hordeum vulgare], two populations were analyzed using 251 genomic and cDNA markers: one population comprised 71 F1 anther-derived double haploid (DH) individuals of an intraspecific cross (IGRI x FRANKA), and the other 135 individuals of an interspecific F2/F3 progeny (VADA x H. spontaneum). The distribution of nonrepetitive clones over the seven barley chromosomes revealed a maximum for chromosome 2H and a minimum for 6H. The polymorphism of the interspecific progeny (76%) clearly exceeded that of the intraspecific progeny (26%) although, based on their pedigrees, IGRI and FRANKA are only distantly related. The contribution of individual chromosomes of the DH parents to the overall polymorphism varied between 8% and 50%. A significant portion (44% versus 10% of the interspecific progeny) of the markers mapped on the DH offspring showed distorted segregation, caused mainly by the prevalence of variants originating from the parent that better responded to in vitro culture (IGRI). In contrast to the interspecific map, probes displaying skewed segregation were clustered on the DH map on discrete segments. The colinear arrangement of both maps covers a distance of 1,453 cM and identifies regions of varying map distances.
Map Data
Barley, IxF
Barley, VxHs
Probe
cMWG684
[ Show all 83 ]
Keyword
Hordeum spontaneum
Hordeum vulgare
linkage map
restriction fragment length polymorphism
Image
1H physical vs. genetic maps
1H vs. rice 5 and 10