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GrainGenes Reference Report: TAG-89-240

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Reference
TAG-89-240
Title
Localization of translocation breakpoints in somatic metaphase chromosomes of barley
Journal
Theoretical and Applied Genetics
Year
1994
Volume
89
Pages
240-248
Author
Marthe F
Kuenzel G
Type
Article
Language
English
Abstract
Karyotype analyses based on staining by acetocarmine followed by Giemsa N-banding of somatic metaphase chromosomes of Hordeum vulgare L. were applied to 61 reciprocal translocations induced by X-irradiation at Gatersleben. Using computer-based karyotype analyses all of the 122 breakpoints could be localized to defined sites or segments distributed over the seven barley chromosomes. Pre-definition of translocations as to their rearranged chromosome arms from other studies rendered it possible to define the break positions even in translocations having exchanged segments equal in size and the breakpoints located distally to any Giemsa-band or other cytological marker. The breakpoints were found to be non-randomly spaced along the chromosomes and their arms. All breaks but one occurred in interband regions of the chromosomes, and none of the breaks was located directly within a centromere. However, short and long chromosome arms recombined at random. An improved tester set of translocations depicting the hitherto known break positions of most distal location is presented.
Germplasm
T1-4al (barley)
[ Show all 61 ]
Map Data
Barley, physical
Rearrangement
T14al
[ Show all 122 ]
Keyword
Breakpoint mapping
Computer-based karyotype analysis
Giemsa banding
Hordeum vulgare
Translocation chromosomes
Image
1H physical vs. genetic maps
[ Show all 6 ]