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GrainGenes Reference Report: INA-11-1067

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Reference
INA-11-1067
Title
Allelic variation at the TaZds-A1 locus on wheat chromosome 2A and development of a functional marker in common wheat
Journal
Journal of Integrative Agriculture
Year
2012
Volume
11
Pages
1067-1074
Author
Dong CG
Ma ZY
Xia XC
Abstract
Zeta-carotene desaturase (ZDS) is a key enzyme for carotenoid biosynthesis, demonstrating high association with the yellow pigment (YP) content in wheat grain. Cloning ZDS gene and developing functional markers are important for marker-assisted selection in wheat breeding. In the present study, the full-length DNA sequence of a ZDS gene on wheat chromosome 2A, designated TaZds-A1, was cloned, with 14 exons and 13 introns, and it has an open reading frame (ORF) of 1 707 bp, encoding 568 amino acid residues. A co-dominant functional marker, YP2A-1, was designed based on the polymorphisms of two alleles at the locus, TaZds-A1a and TaZds-A1b, yielding 183- and 179-bp fragments in TaZds-A1a and TaZds-A1b genotypes, respectively. A new QTL for YP content was detected on chromosome 2A, co-segregating with the functional marker YP2A-1 and TaZds-A1; it explained 11.3% of the phenotypic variance for YP content in a doubled haploid (DH) population from Zhongyou 9507/CA9632. Among 217 Chinese wheat cultivars and advanced lines, the average grain YP content of 126 cultivars with TaZds-A1b allele was 7.8% higher than that of 91 cultivars with TaZds-A1a allele.
External Databases
http://www.sciencedirect.com/science/article/pii/S2095311912600999
Gene
TaZds-A1 (Triticum)
Locus
TaZds-A1
Probe
YP2A-1
Keyword
functional marker
QTL
Triticum aestivum
ZDS
Zeta-carotene desaturase
Summarized in
ReferenceMAS Wheat. Functional Markers for processing quality genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.