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GrainGenes Sequence Report: BE426287

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Sequence
BE426287
Contig
TaAffx.92142.1.S1_at
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC305459
wEST map position
BE426287
NCBI UniGene
Ta.38412
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, weakly similar to NP_188445.2 unknown protein [Arabidopsis thaliana]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3AS
3DS
Clone Library
Wheat unstressed seedling shoot cDNA library
Tissue
Etiolated shoot
Developmental Stage
Five day old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0330_E10_I20ZS Wheat unstressed seedling shoot cDNA library Triticum aestivum cDNA clone WHE0330_E10_I20, mRNA sequence.
Other Name
WHE0330_E10_I20ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA006E1X
Clone
WHE0330_E10_I20
Probe
WHE0330_E10_I20
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
TaAffx.92142.1.S1_at900WHE2AFFY
TaAffx.92142.1.S1_at694WHE2AFFY
DNA
atcagtcttactgtcaaggttggtttggtgctgtttgcagtatgcattct
cgcgccggtttcgctcatggtgttgctcaggcacgcggttcctctgcaga
cattgaggttgctgttttcagctggttcagcatctttggtggcttgggaa
gaagtaaagatgtactctgcaaacaaaggagcgtttctgtgtgagtgtga
catgtcgagcctaagatcagatgtatgcgagctgaagggcgatgtccggg
taatcccctcaaacatcaccatcattgccctcctccacccctctgtctct
gagagtcgccggtcatggaggatgaagccgcacgcgaggaagaacgatgg
ccacgcgctcgccagcgtcacagagggtgttggtgtcggtcaccccttca
tcacctcacgttccggaatgcaaggccgagagtgccgcccccgcggtggt
cttctcagtgggcgcctatgcaggcaacatgttccatgacttcaccgacg
tactcatcccgctcttcatcaccgccagccggttccgcagcgacgtgcac
c

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