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GrainGenes Sequence Report: BE516501

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Sequence
BE516501
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DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Germplasm
BREVOR
Species
Triticum aestivum
Cultivar
Brevor (soft, white, winter, common wheat)
Clone Library
Wheat ABA-treated embryo cDNA library
Tissue
Seed embryo
Developmental Stage
Mature dormant seeds
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE611_E09_J17ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE611_E09_J17, mRNA sequence.
Other Name
WHE611_E09_J17ZA  [ wEST-ACEDB ]  [ wEST-mySQL ]
Strain
lab_host E. coli DH12S
DNA Library
TA012XXX
Clone
WHE611_E09_J17
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Clontech Matchmaker 3' AD primer.
Note: Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
gcggccgcgtcgacccacggaggagtattataacaaactgcatgagacaa
caaagtgagcttcaaagcgacatctaaagtgctgcaccattgtcgtcggt
gagcagaaacaaaataatttgacgagtactgcaccactttttcctagtgc
attgcattataaaactaacaacgattttgcgagtactgcaccacttttta
tgagtgcactgcatgactaagtataaaattaacgaccagatagcgtcatg
caatttttctgaaaatggaaaaacaatcaacttcacaacagccaccggca
acccacgcgccgtgtgctgcatacacacacgcgctgtactgcatggacgt
gccagcggactgcacggagggaggctcgctgtccaaactgtatgatcagg
gaaacagaaaaaaaatgcaacgctgcatcaaaccgcactctaccactttc
ttcctgcaagaacaagtattgaacctcatgagcaaacatgtttctcgtca
ggggacatgagcgggagatgcataaggaaggagaagatgcaattcacaac
atagtatctcgcaaacgaaccacgtgccgagctcatccctggtgcatcaa
tggtcgagggatccggcgcctggcgt
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