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GrainGenes Sequence Report: BE516639

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Sequence
BE516639
Contig
Ta.11.2.S1_a_at
Ta.11.3.S1_a_at
NSFT03P2_Contig17478
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC311052
NCBI UniGene
Ta.11
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Cathepsin B'
Keyword
EST
Germplasm
BREVOR
Species
Triticum aestivum
Cultivar
Brevor (soft, white, winter, common wheat)
Clone Library
Wheat ABA-treated embryo cDNA library
Tissue
Seed embryo
Developmental Stage
Mature dormant seeds
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE618_B08_C16ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE618_B08_C16, mRNA sequence.
Other Name
WHE618_B08_C16ZA  [ wEST-ACEDB ]  [ wEST-mySQL ]
Strain
lab_host E. coli DH12S
DNA Library
TA012XXX
Clone
WHE618_B08_C16
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Clontech Matchmaker 3' AD primer.
Note: Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tcgcggccgcgtcgacgaaagatatcatacaaacggtcaacaaccacccc
aacgccggatggacggctggacacaacccctacctcgcgaattacactat
tgagcaatttaagcatatgctgggagtgaagccaacgcctccaggtttac
gggctgctgttcgcaccaaaactcattcaagatcggagcagctcccaaag
gtgttcgacgccaggtcgaaatggtccggttgcagcacaattgggaaaat
acttgatcaaggtcactgtggttcctgctgggcttttggtgccgtggagt
gcctgcaagatcgtttctgcatccatcacaacatgaacattacactttct
gccaatgacctagtggcttgctgtgggtttatgtgtggcgatggttgtga
tggaggatatcctatcagtgcgtggcaatacttcgtccagaatggtgttg
ttactgacgagtgcgatccatactttgatcaggttggctgcaagcatcct
gnatgtgaacctgcttatcctacacctgtgtgtgaaaagaaatgcaaggt
gcagaaccaagtttgggaggaaaagaaacatttcagcatcaatgcatacc
aagtaaactctgatccacatgacatcatggcagaggtctacaataatggc
cctgtagaagttgctttcacagtttatg
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