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GrainGenes Sequence Report: BG262775

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Sequence
BG262775
Contig
Ta.9535.4.S1_x_at
NSFT03P2_Contig17444
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC303881
wEST map position
BG262775
NCBI UniGene
Ta.54428
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001056190.1 Os05g0541900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3BL
3DL
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0945_B04_C07ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0945_B04_C07, mRNA sequence.
Other Name
WHE0945_B04_C07ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA018E1X
Clone
WHE0945_B04_C07
Probe
WHE0945_B04_C07
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.9535.4.S1_x_at1104WHE2AFFY
[ Show all 13 ]
DNA
ggcggcgcgtcactccaatcaccggttaggataaatggcgacggtttcgg
attctctggtctgggagctcgtcaggaagaacaactgcttcctgatcaag
cagttcggcaacagcaatgccaaggtccagttcagcaaggagcccaacaa
cctctacaatgtccactcctacaagtactctggcctggcaaacaagaaga
ctgtgactgtgcagccagctgctgataaggagatgaccgtggtcctctcc
acaacgaagtcaaagaagcagaacaagcctgctgccttcacccacaagac
tgtcatgcgcaaggagttccgcaagatggccaaggctatcaagaaccagg
tcagtgacaactactacaggcctgacttgaccaagcctgctcttgcaaga
ctgagcgctgtttaccgcagcctccaggttgccaagtccggtgtcaagaa
gaagaacaggcagtaagcactgaggggccaatgagcttgttttgtggatc
tgttggtttatgtttccagctttttagatttgttctaagagtatggagat
tgaatatgat

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