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GrainGenes Sequence Report: BG314580

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Sequence
BG314580
Contig
Ta.2282.1.S1_a_at
Ta.2282.1.S1_x_at
Ta.2282.3.S1_a_at
NSFT03P2_Contig11946
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
DB Remark
Locus Source: Triticum monococcum
Keyword
EST
Species
Triticum monococcum
Cultivar
DV92
Clone Library
Triticum monococcum early reproductive apex cDNA library
Tissue
Early reproductive apex
Developmental Stage
Seven week-old plants
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2488_G07_M14ZS Triticum monococcum early reproductive apex cDNA library Triticum monococcum cDNA clone WHE2488_G07_M14, mRNA sequence.
Strain
lab_host E. coli XLOLR
Clone
WHE2488_G07_M14
Remark
DB_xref: taxon:4568
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
taatgcaatgaatgagcctggacatcatggaagcaactctttctttgggt
cagatgacttcaatgtagaccctgttagagtgggctctccaagtggcgct
agtgcctatggaaagaagagcacattctttgacgattccgttccaagttc
ccctgcatacacgtctgggttctcaccgaagttcggtgaaagtcgtgacg
atagctcctcctataactttggaaggttcgattccttcagatcccaagac
agcgccgtcccacaggaaagccgcttttccaggtttgactctgtaagcag
ctccaaaggcgagagcgcagcgggatttgactcatccaacagctcgagaa
atttcggccggttcgattcttttgatgaagctgatcctttcggctcgact
ggaccttttaaagcatcaggagcgcggtcgccccccaaattctgatattc
atttgtggtattacgaggatctgtctcttgcgggctcatctgtcgataca
aaggctcggattgttcgaggaaagtacaatacagcggatccatttttttt
ggttggcaagtctcttgtgttggccttgtgtgcatttgtcctgtcctcca
ccccccacg

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