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GrainGenes Sequence Report: BM140556

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Sequence
BM140556
Contig
NSFT03P2_Contig13646
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC329351
wEST map position
BM140556
NCBI UniGene
Ta.56209
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001049502.1 Os03g0238800 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Chromosome
4BL
Clone Library
Wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0485_d03_h05zS Wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone WHE0485_d03_h05, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0485_d03_h05
Probe
MAG3078
WHE0485_d03_h05
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20. No effort was taken; to identify ESTs of fungal origin from this library, thus this EST; could be of wheat or fungal origin.; Seq primer: Strategene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made , and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tccttctatgccctggggtccaccatcaatgccagctaatgtgaggccac
attcaccaagtccccagcacttgggatacaccccgaccagcagcgtactt
ccagcaccaacaactggacctcatcacatccctccgtctgatggaatgca
accactcttagtagcacctgctcctgtcgctgccgcagccatacctatcc
cagcagctgttcccttgccaaatgcaacggcagcttggatgccagaagct
gccccaaggcctgccccacctcgatcccctgtgccaggcacaggcgtctt
ccttcctcctgtatcagctcaccagctgccccatc

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