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GrainGenes Sequence Report: BQ161251

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Sequence
BQ161251
Contig
Ta.8085.1.S1_at
NSFT03P2_Contig2701
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC317368
NCBI UniGene
Ta.8085
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, weakly similar to NP_001065009.1 Os10g0505900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat cold-stressed seedling cDNA library
Tissue
Seedling
Developmental Stage
Five-day old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0369_B04_D07ZT Wheat cold-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0369_B04_D07, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0369_B04_D07
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttttttttttttttttagacgaagaaaatcgtgt
ttgaactttgaacacacagattacacaggaagttcagaagaacttaaaaa
gacaaagacagaaacatgaacatacacacgcgtaccgtacatgatttaca
gaacacacaaaacgtgtgaaacttacacgaacacttctggaacacaagcc
ccaaatacatacacgaacgataaaagcagctaagccccagatgaacccaa
acaccagtcagtgtacacctgaaccagccaggcgacttattccagcgatc
ccacgaactgatcacacgctgctgcacttcacatcacattcgtgttggtc
ctcggagcctcgccgtgaagcaccggcccctccgccgcgacgccctcccc
gatgttcccctct

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