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GrainGenes Sequence Report: BU987930

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Sequence
BU987930
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC154186
NCBI UniGene
Hv.20839
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf79h08, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HF
Tissue
caryopsis
Developmental Stage
developing caryopsis, 16-25 DAF (days after flowering)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HF16C20r HF Hordeum vulgare subsp. vulgare cDNA clone HF16C20 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HF16C20
Probe
GBR1303
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 663 Std Error: 0.00; Plate: 16 row: C column: 20; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); developing caryopsis , 16-25 DAF(days after flowing) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 940 bp
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); developing caryopsis, 16-25 DAF(days after flowing) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 940 bp
DNA
caaagcatcagtatgactgggatgttgcaactacattctctttcctacag
aagtttggtcttacaggggaattcaagataaatgttgagtgcaaccatgc
cactctctctggacatagctgccatcacgagcttgagactgcacgcatta
atgacattcttggaaacattgatgcaaacactggtgatccacaagttggt
tgggacacggatgagttccttacagacatttcagaagctaccttgattat
gtcaagtgtagttaagaatgatggacttgcgcctggtggcttcaactttg
acgccaaattgcggagggagagtactgatgttgaggacctgtttattgcc
catatctctgggatggacaccatggcccgcggcctccgcaatgttgccaa
gctgattgaggatggttccctggacgagcttgtacgcaagcgctaccaga
gctttgacactgagattggtgccatgatcgaggctgggaagggcgacttt
gaaacgctagaaaagaaggtcttggagtggggcgagccaaccgttccatc
gggcaaacaggaactggctgagatgctattccaatccgctctgtagatgg
cggcccgtgattctaggaataaaaaagcaagagcgcgaccttggaacgcc
cggccgtcctcgt
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