|Locus Source: Triticum aestivum (bread wheat)|
|UniGene title 'Transcribed locus, moderately similar to NP_001053208.1 Os04g0497700 [Oryza sativa (japonica cultivar-group)]'
|Triticum aestivum FGAS: Library 2 Gate 3
|FGAS052966 Triticum aestivum FGAS: Library 2 Gate 3 Triticum aestivum cDNA, mRNA sequence.
|Feature: source: mol_type = 'mRNA'; |
|Feature: source: mol_type = 'mRNA'|
|Locus Comment: Contact: Wm L Crosby; Bioinformatics; University of Saskatchewan, Department of Computer Science; 1C101 Engineering Building, 57 Campus Drive, Saskatoon,; Saskatchewan, S7N 5A9, Canada; Tel: 306 966 1769; Fax: 306 966 2033; Email: email@example.com; This sequence is the direct result of the Base calling software; Phred (default parameters). It is the raw base calls. To aid in the; identification of the high quality insert the software Lucy; (default parameters) has been run on this sequence. Lucy identified; the region [99,583].; Plate: WEF001 row: E column: 11.|
|Note: Organ: Crown and leaf; Vector: pCMV.SPORT6; Aerial parts (crown and leaf) of wheat cultivar Norstar from control and long exposure times to low temperature. 4 mRNA populations were combined before constructing the library; 7 days non-acclimated plants and 1, 23, and 53 days cold-acclimated at 4C. Non-acclimated and cold-acclimated plants were grown in vermiculite This is the only library that was done according to the Invitrogen manual, and therefore, a percentage of clones will not have the 3 prime end because of NotI digestion within the cDNA.