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GrainGenes Sequence Report: AZ048449

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Sequence
AZ048449
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
Keyword
GSS
Species
Hordeum vulgare subsp. vulgare
Cultivar
Betzes
Clone Library
Barley PstI genomic clones
Tissue
Leaf
Data Source
genbankRelease 135, Apr 15 2003
Title
PSB67 Barley PstI genomic clones Hordeum vulgare subsp. vulgare genomic clone PSB67, DNA sequence.
Clone
PSB67
Remark
DB_xref: taxon:112509
Locus Comment: Contact: Laurie DA; Cereals Research Department; John Innes Centre; Norwich Research Park, Colney, Norwich NR4 7UH, UK; Email: david.laurie@bbsrc.ac.uk; Mapped on chromosome 1HL. Second copy detected on chromosome 6H; using wheat/barley addition lines.; PCR PRimers; FORWARD: M13 Forward; BACKWARD: M13 Reverse; Insert Length: 1289 Std Error: 0.00; Class: shotgun; High quality sequence stop: 1289.
Note: Vector: PUC18; Site_1: PstI; Site_2: PstI; Genomic fragments from PstI digest of DNA extracted from leaves.
DNA
ctgacatgaccctcggatatttatgttttagggtatgcaatccatctact
tctcgcagttctgaaatgtacccctgtcaatacttgtagttgcagacttg
cagtgccgaaaattcacagaagaatacatagtgaagaacttacatacttt
ctgtacattttactccccctattcatactcatactaactgtaaaataata
aaatacagtaggttatccttttgtttcttatgggcctttggggtttgttg
acattctggcatttaatctttatcacaataagctgtattttaaatcagtt
tgtttcagtgaacatacaacattcttatgtcctttttctatccctcgcac
agttttcaggaaatagtccctctcaatgccggcaatgtgcttgtgataga
agacaacgaaccagctgcaaggtggcttgctcttataaaccgcacgctaa
acagaccagttgatggggatgctgatatcttcagtcacaagccatctcta
tcccttgattcaacctcgtctcgatcttcatcaaaccttgacgcttcttt
ctccaaccgctctggttctgcaatcttccagtcctccttaaaatctatca
ggaagccttacatgccaactcagagaaaactgctcaaaatctgcaactgc
tcagttgaaatgcccaggaaatcctacaaagatgcttgctttggatgccc
acaagcatacattaatgagaccgactctcagaagatgaagtgatgacaga
tcgaataatgcttctggtatgtgtgatggagtgatttcgtctgcttctgc
ctctagcgaccagctaaaatataacctcgtatcttgcaaacgaatggttg
gtatttttgttacagtatgggcaaaaaaagaactagtgccgcatatcggt
catgtgcggacatcatgtgtaggccgtggggtaatgggttatcttggaaa
caaggttagaaccgagtcaatattcaagtaattccttttgattatgcaca
ttgtagcatgccctaattttttgttgaaccgttatgtaagtcggaaacaa
ggttagtaccaggtcaatgttcaagtaattccttttgattatgcaaattg
tagcaagctctaagataaaatgttatgatagtcacgaagacattaatgaa
tgcagttatgtagtactatgctcatgctatttttctcatatcaacttctg
tcggaataattacttgctgcttgccaaaaaaagcagaatatgaatgaaaa
attctgaagcatcctgaacggtaggatcccttatcctct
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