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GrainGenes Sequence Report: AZ048454

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Sequence
AZ048454
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
Keyword
GSS
Species
Hordeum vulgare subsp. vulgare
Cultivar
Betzes
Clone Library
Barley PstI genomic clones
Tissue
Leaf
Data Source
genbankRelease 135, Apr 15 2003
Title
PSB95 Barley PstI genomic clones Hordeum vulgare subsp. vulgare genomic clone PSB95, DNA sequence.
Clone
PSB95
Remark
DB_xref: taxon:112509
Locus Comment: Contact: Laurie DA; Cereals Research Department; John Innes Centre; Norwich Research Park, Colney, Norwich NR4 7UH, UK; Email: david.laurie@bbsrc.ac.uk; Mapped on chromosome 7HS. Additional copy detected on chromosome 6H; using wheat/barley addition lines.; PCR PRimers; FORWARD: M13 Forward; BACKWARD: M13 Reverse; Insert Length: 1439 Std Error: 0.00; Class: shotgun; High quality sequence stop: 1439.
Note: Vector: PUC18; Site_1: PstI; Site_2: PstI; Genomic fragments from PstI digest of DNA extracted from leaves.
DNA
ctgcgaggttgctcctggtgtgctcagttcttgtgctgtgccttgtaagc
ggaggcgcgaggtgtgacaagttaagcagcaatttctacagttggacatg
ccccggagtgtacaacatcgtccagcagcacgtgttttctgccgtgaggg
aagagctgaggatgggggcctccttgcttaggctccattttcatgactgc
tttgtcaatgtacctcaagtttcttctgaacttactatgaatgttatgtt
gctgtccatctactattgtgttgcagttactgaaaaagaaatttgtgatg
tattcagggctgtgatggttcaatcttgctggatggtgctgatggcgaaa
agtttgcactacccaataagaactccgtcagagggtacgaggtcatcgac
gcaatcaaggccgatctcgagaacatgtgccccggggtggtgtcctgtgc
tgacattgtagcccttgcagctggctatggagtactctttgtgagcatct
tacatcttgtgctccattttgactgatacattcagtttgtcgtacaacgt
attgagtaactgtgcactgatttattttagagtggagggccttggtataa
tgttctactggggagaagggacggtctgaaggcgaatcagtcaggagctg
acaacgggctgccctcgccgtttgaaccgatcagctccatcgtaaagaag
tttgccgatgtcggcctcgacacgaaggatgtcgtggttctttcaggtaa
cgattgtcagactgatcacaaaactaactacctccacgaaagttcacccc
tagaattaaatcccgtgctacatccctgaaaatgaaattgtgactgtgtg
gccatgcctagctcgagcagaagccattattgtcagttctggaacatcat
tcctcacatgcaagctgtcacaaaattttcttaggctaattatcatacag
ttgaccagatcttatggaatggagtgcagaacgaaaaactctgtaatttt
gtcaaagaaacaggcatgctctacattgtgagagtgtggccatgctactc
agagtagtagcaacaactagtagttgtgaaacttcactcctgacaagcaa
antgtcacaaaattttcttaggccaattctaatcactttgattatcgtac
agttgacctgatcttatggaatggagtgcagaacgaaaaactctattttg
tgaagatagaacacatgacctaaattttgaacgccaaaacctgttgagtt
atttaggaaatactatatgatagtgtaaaatttattctactttgtcttgt
acttcaagaaggtcatgtactttttatacatacgccatctaggcttagca
ataaacaacaattccatcaatttcccttttaatccctcttacatgatagg
tgcccacacgatcgggcgagcccggtgcgtgttgttcag
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