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GrainGenes Trait Study Report: Diaphorase activity, Souza88

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Trait Study
Diaphorase activity, Souza88
Trait
Diaphorase activity
Reference
ReferenceSouza E (1988) Measures and applications of genetic relationships in oats.
Protocol
Diaphorase and esterase stains were assayed on horizontal slices of as single gel. Diaphorase activity was assayed by incubating gel slices at 37 deg C in 50 ml of a solution of 2.0 x 10^-4^ M 2,6 dichlorophenol indophenol, 4.8 x 10^-4^ M 3-(4,5-Dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT), 2.0 x 10^-4^ M NADH, and 0.1 M Tris (Weeden, 1984). Diaphorase enzyme activity was found in six well defined zones at Rf 0.82, 0.79, 0.76, 0.29, 0.25, 0.21. Additionally, there was a zone of diffuse activity from Rf 0.60 to 0.45. Five of the six zones were invariant in the cultures surveyed. However, the isozyme at Rf 0.76 was not present in 17 of the 70 cultivars. The null zone was found in 59% of fall planted cultivars while only 3 spring cultivars were found to be homogeneous for the null zone and a single Spring cultivar, multiline Multiline E77 (oat), was heterogeneous for the null phenotype. Dal was the only recent spring cultivar with the null Rf 0.76 isozyme. The other two were Green Mountain and Joanette, a historical cultivar and a parental source of stem rust resistance, respectively. Crosses between Ogle (Rf 0.76 isozyme present) and two parents with the null allele were examined for segregation of a null allele at the Rf 0.76 zone. In the Ogle/White Lustre and the Ogle/Windsor crosses, the segregation for the null allele fit the 3:1 segregation of a dominant gene at a single locus, designated for this paper, Dia-1 with alternate alleles Dia1(present) and dia1 (absent).
Values
Dia1 = active allele
dia1 = null allele
Germplasm Description
Advance (oat)
Dia1   active allele
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