Microsatellite polymorphism in a sample of barley germplasm

John Korte, Zhaowei Liu, M. A. Saghai Maroof, and Patrick Hayes

We have generated data on microsatellite, or simple sequence repeat (SSR), polymorphism in a set of barley genotypes . These genotypes were of interest to us in our barley stripe rust (Puccinia striiformis f.sp. hordei) resistance breeding effort. Our plan is to use SSRs as framework markers, together with higher throughput markers, like AFLPs, to quickly generate linkage maps that can be used for QTL detection. We used the commercially available Mappair primer sets that were developed by M. A.Saghai Maroof and his group (see Saghai Maroof, et al; 1994; Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations, and population dynamics. Proc. Natl Acad Sci USA 91:5466-5470 and Z. Liu, et al; 1996; Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theor. Appl. Genet. 93:869-876). Microsatellites have been technically easy to bring into the lab (using the accompanying protocol). The data we present were generated at Virginia Polytechnic Institute and State University and Oregon State University. We scored, between VTI and OSU, a total of 24 different genotypes. Three genotypes, Kold, Colter, and Cali-sib, are common to both data sets. We present two sets of autorad images, raw data matrices, and cluster analyses due to the independent scoring of alleles in the two sets of genotypes at the two labs. (VTI used 39 primer pairs and OSU used 27 to gather the accompanying data). The presence of the three common genotypes should be of assistance in identifying polymorphisms between other genotypes that were not in common. OSU-Barley








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