INRA CLUB SSR-Screening

Varieties included in the screening :

SSR Primer Pairs

Name

Leftprimer

Tm

Rightprimer

Tm

gpw93012

TTGGGAAGAGGACCAGGG

61.0

GCCATGAACTGCTGGGAG

60.4

gpw93014

CCCCTGAGAACGAAAACAAA

60.1

TGGGTCAACTATGTGTGGGA

59.8

gpw93020

CTCTGCTTCGTGTCGCAGTA

60.3

AGTTCATCGGTCGTTGCAG

59.8

gpw95003

TCATTCGCTCATACGATAGGG

60.1

CTGGATCACCTACTTTTCTCCG

60.1

gpw95011

CCAAACAGCGACATTGAGC

60.4

AGAGGTTGAAGACGGTGGG

60.1

gpw95017

AAAGAAGAGGGGGGACCAC

60.3

TTGTCAGCGGATTGGACTC

59.8

gpw95019

ATTTGACGGACTGACAGCG

59.8

CTGCTTCCAAGGATTGTTTTT

58.4

gpw95023

TGAGCTCCACATCTCCAT

63.0

CAACTCCTCTCTCACTCATC

61.0

gpw95026

ATGGGCCTTTAAGAGCAAAA

58.8

AGGGGTGAAAGGTTGGAGAC

60.3

gpw95028

AGTATCGCAACGGCAACAG

59.9

ATTTCACTCCCGCATTTGAC

59.9


PCR protocol
· used in Clermont-Ferrand Tm : 60C

PCR Profile :

PCR Conditions

 
 
 
 
>gpw93012
TTTGGGAAGAGGACCAGGGCCTCTATAAATAGGATCAGCCACCCGCAAGACACATGGATG
ACGACGGACGACGACGACGAC
CACAGACACACACAGGGACAGAGAGCTGAGGGGTTCGGA
GAAGGAGGCTCTTAAGAGAGGGGTTTAGATC
ATATATATATATATATATATATATATATA
TATATATATATATATATAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
AGGGTGAC
TGAGCTCCTCCCAGCAGTTCATGGCC
 
gpw93012
 
 
 
 
 
 
>gpw93014
GTCGCAGCGCCCCGCCGCCGGCCTGCTGCTGCATCCACTGCAGCTCGCCCCCGACTGGCT
GCCGGCGCCCACGCCACGGGCCGCGTCCGAGGCCAGCCCCGTTCGGCCTCGGGCACGGGC
ACCACGCCCGCACCCAGCGCCCGACCCGCAAGGCCTCTGTGCCACAGACAACGGGGCCCA
CCCCCTGAGAACGAAAACAAAAAAAATAATACTAATTAAATTAATTTTTTAAAAAATGAA
TTAATAAATAATAACTAAAATTAATTAAAATGAT
AATTAATTAATTGATTAAAGGCTTAA
TTAAATTAATTAGTCTTGATTAGATTAACCTAATTACTAGTTAACTTAATTAACCCCCTG
ATTAGACTAATCAATCAATGACATGCGGGTCCCACACATAGTTGACCCAGTCAACTCTGT
TGACTGCTGACGTCAGCATGACATCATGCTGATGTCATAAAACCAAATT
 
gpw93014
 
 
 
 
 
 
>gpw93020
AGGTGCTGTCGGGGTTGCAGTTCTGCGGGTACGACCCGTCGTCGCGGTTGGGCCACAGCC
CGTGGATCCCGAAGTCCGCCGCCGGCTTGCCGGACCGCGGGTAGCAGCAGCTCTGCTTCG
TGTCGCAGTACGACCCCGGCCACTGC
ACACACACACACACACACACCAAGGCCAACCGCC
GGCACGAACCATCCATCAGAAACCAAGCAGCACGTACACCACGTCTCGGCAACTGCAAGG
CTGCAACGACCGATGAACTGAACCGGCCACACGCGCACTCACCTGCAGCACGAGGTAGAA
GAAATCGTAGCCCGCATGTTTCCCGCTCTCCTGCGCTGCCGCGCAGCCGACGCTCAGGCC
GATCAGGAGGGCCAGGGAGCACTGCAACTTCATGGCTTTGGATGTCTTTGCTGGTTGTAT
TGATGGTTCTTCGGAGATGAGATGAGATGAGATGGAAGCTTCCGTTGGTGTTGCGGATTT
ATAGGCTGCGTTGATGTGGATCTACTCCACGGCAAATGCCAGGTTTCCGGAACGTGCATT
CCAAGGAGGATTTAATTGCTTGCACTTTTGGGCTTGCGGGAATGCCAGAGATGGAAGCAT
GGCTGTGGGTGGCGAGGTGTGCAAGCCAAGCAGCCGAACCAATTGTAGAATCCACGGGTC
CAGCCATAAAAAGATAGCATACACAACTACTAAAACATACAGAAATCACCAAGGAATAAA
GAGCTTTGCTACACATACATAATGTTACAGGGATGAGGGGTTTACTGGGTGTAGAGGCAC
AGGCCCACCCGCATTAAAATCAGGGAGATGTGTTTAGAATTTTTTTT

 gpw93020
 
 
 
 
 
 
>gpw95003
GTGCAGCACATTTTTCACTTTCAGGTCTTTTTTTATCTGCAAAAGAAGAGAACATATTAG
AGCAGCTGGCTGTATAAGATTGCCGTGTCATCAAGAGGTACCAATATCATTCGCTCATAC
GATAGGGTTGGCTGGCTAGTGATATTT
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT
CTCTCTCTCTCTCTCTC
TTTCTATTTCCTCTCATTTAACTAGGAGCACGTGAAGAGCTTG
GGCATTTGTTGCCTTCCACTATGTGGCCGATGCCATATTTCGCACCGCTCTCGCGATGTC
AGAGAGTTGGCACCGCTGTGCACACAGACCCACATGTATAAACAAATCAATTAAACCAAC
TACACCAGCCTCTCACTCTCCCAAACAAGTGTTTTTTTATTGCCTCAGTCCTCTCTCTCT
CCCACGCAGTGTTTTTTCATTGCGTGAATTAATTTGGCACCGGAGAAAAGTAGGTGATCC
AGATTGGGGCACCAGGTGAGCAATGGAGGGGC

gpw95003
 
 
 
 
 
 
>gpw95011
TCAACGTTCTTATGAGGTGCAACCTGGATCGTGAAGATTGTGTTAGTTTGCATTCCCCCT
TCATCTTACCCTACGCTTGAATCTCGGGACGAGATTCTTGTTTATTGGGGGTGACTTGTC
ACATCCCTAGCTTCTGGCAATGCACTAGGCTAGCCTCATGAGTGTCATCATATTTAAGTT
TCAAATGAACTTGAAATGGGGATTGTTTAAACCCTAGCATCAAAGGAATTCACTAGGTTC
AACTAAAATATTCTTAGTGAGTCCAAATGGCTTTCAAAAATGTTCATCGTTTCCGGAAAA
TGCTAGAAGCAATAACCAGAGGTGTTGCACATTTTTCCATGACATATTTGGGCTCTGAAT
TAAATCATATAGTATTTGCATTTGGGCATATAAATGCTATTAAATATTTTAAATGCTCAA
ATAACCATAAACTAAAATGTTTGCTGTTGGCTATATTCCAAACAGCGACATTGAGCAGTT
TCATGATTTTTGGATCTGTTTAAGTATTTTTAATAAAGCCCTAAAGACTACAGAATAATA
GAAAACAGAAACTAAACAGGAAATAAAA
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGACT
TACTGGCTTACCTGCGCAGCCCACCTGGCGGCCCAAGTGGCCAGCCCAGCCCACTGGCCT
GGCACTGTTGGCCAGCCCACCAGGAGCCCCACCGTCTTCAACCTCTGCTAGTAGGCAGAG
GCGT

gpw95011
 
 
 
 
 
 
>
gpw95017
CAAAGAAGAGGGGGGACCACCCCGCTGATTGATAAGGCGCCCTCTTACTTATATACTACT
CTAGACTAAAAGTGTGCAT
GAGAGAGAGAGACAGAGGGAGAGAGAAAGAGAGGAGGGAGA
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGACGAGAGATAGTGGAAAAAGAGTTGCA
ACATACAGTGCAAGTAAGAAAGTGTGCAGGGAAGAAAGGAAAGCGGAAAAAGAGAA
GAGA
GAGAGAGAGAGAGA
GTGAGAGAGAGAGAGAGAGAGAGAGTATAATATTCCACGAGTCCAA
TCCGCTGACAACTTTTAGTAATGTGACACCACGTCATCACCCGGTCATTATTTCTAACCG
TTTTTCGGCCTGTCGCT
 
gpw95017
 
 
 
 
 
 
>gpw95019
CCGGTTTGTTACGCGGTTTAAACGGGATTCGCCTGCTGTTAAATTTGACGGACTGACAGC
GCGGCGCGGCAGATAGAGAT
AGAGAGAGAGAGAGACTGCCGCGCGGTTTAAACGGAATCA
CTTGCTGTTAATTTGACAGCTAGCGTGGCGC
AGAGAGAGAGAGAGAGAGAGAGAGAGAGA
GAGAGAGAGAGAGAGAGAGAGAGAG
TTGTACAGAAAAAAAACAATCCTTGGAAGCAGATC
TCAACCGGATCTG

 gpw95019
 
 
 
 
 
 
>gpw95023
ATGAGCTCCACATCTCCATGGATGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGAGGAGGA
GGAGGAGGAATATGTATTGCAGTACAACAACTTTCCATCTTTTTCCAAAATGAGCTCCAC
ATCTTCATGGATGAGTTCTATCAAGATATGCACATGAACTAAGTCGCAAGAACTAGAAGC
AAGATGAGTGAGAGAGGAGTTGCCGGATGCCTATGTTTGAGTTGTTCTTGACAACACTCT
TTGGCCCCTAAGTAATGGCGTTGCGCCAGCCAAAAGGTGTGGCTATGGTTGAACACAACC
AAGCCCCCAAGTGACTTACTCTCGTGGCTTTTGAGCCAGCCAAGAGGTGTAGCTATGGT

 gpw95023
 
 
 
 
 
 
>gpw95026
ATCAGAGATTAGTATTACTACTGGTCAAGAGATTAGGTACTAATGGGCCTTTAAGAGCAA
AATAAATGAAAATGACTCACCCTAAAATCAGGCCAAC
CTCTCTCTCTCTCTCTCTCTCTC
TCTCTCTCACACACACACACACACACA
TTCACTGCATGGTTCTTAGTAGAGCGATCCGAT
GGCATGTGCTCCCATTCATGGTCTCCAACCTTTCACCCCTCTAATTAGTTTCTACTATGA
TAATATTTGTTCTTTCTGATACTTCATTAATATTTTCTTGTTGGGTAGATTTACATTTTA
AGATAAAATGTGAATTCACTGAAAAAATCTGTAGCATCAGGATAATTTATACAAATATTT
TAATTTATATTTTTTAAATAAAGGGCGTAATTTTCATTTTGCACGGGGCCCCAAATTTTA
AAGGTACGGCCCTGATCAGTCACATCAGAATTGGAATGTAAGTTAGTTTCACAGTGATGT
TGATTGAAGGATCATCCAATAATGCAAAGTTCCTTTTTGTCTGCAAACTTATTTGCTTCT
TAAAAAGAAAATAATTGTGTTTTTTTACCTCACCTCTCCCATCACAGAGAAAAAAAAAGA
CAATCCTTCCAAGGACTGGTTCAAACATTTTTTTTATCGGAATTTGCATCTAGGAGTAAT
AAATAAAAAGATAAGCAATTATATATTTCAAACTTAAAATTTGAGAGCCAGGTAGTTGG

 gpw95026
 
 
 
 
 
 
>gpw95028
GCTCCACGGGAAGTGTAGCCGGGCTTAGGCTGCAACGCTCTGGAGTGGGGGATGGGGATG
GGGATGGGGATGGGGATCTGTGGGAAAAGGGGCGTTTGGCAGGCGTCATCTAAGAAACTC
AGGGCGGCCTTGGTATGGAGATCAGTGGGTAAGCTGCACGGGCAAACCGGCAGATGTTGA
CAATGGAAGTATCGCAACGGCAACAGCGGCGGCGGGGACCTTGCTAGGGTTTTGACCGAG
GGAGGGGGTGTGCGTGTGCACGCGCCCGAGGAGGTTTTA
GTGTGTGTGTGTGTGTGTGGA
AGGGGGGGGTGTTTGAGGAAATGACGAGGGTACTAAATTTTTTTACTACGCGAGAGTCAA
ATGCGGGAGTGAAATGCCGGGGCTATTGAAAATTTTGATTATGTTGCATCGCACACGGTC
CGGAGATAACAACTGTGTGTTATGAAATAGAAAAACCCTTGAGGCAGGCATATATTTTCT
AGGGGCAGGTGGGATTGGGAACAAGAAACATCACACACGGTCCGGAGATAACAACCATGT
GTTATGAAACAGAAAAACCC

 gpw95028