gpw93003
CAAGTGTCATGACTTTCAATCCTCTGCATCATATGAACACTAAAGAATTCGGCACCTCAG
AAGAGATAGAGATGAACATGTATGATCATTGGATAGACACTTATGATTTCTTTGAGTAAA
GGGCGGTCAATGACTGGTATTGACCAAGGGTTCCCCTATTTAGTTGTTATGATCTCTCCT
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCGTTGTAAACTCTCATCATGATTAGTGGAT
GGTATCTTCCTCTTCTACAAGTTGTAGTAACTTAGCTTTAGAACCCTAGAACTACTTGGT
AGACTAAGGTGATCTCTGAAACTTGTATAAAATTACCAAATCGGGGAATATGTTGTTGGG
GAACGTTGCATGAAACCAAAAAAAAATCCCACGCCCACACAAGATCTATCAAGGAGATGC
ATAGAAACGAGAGGGGAGAGTGTGTCTACGTACCCTTGTAGACCGTAAGCAAAGCGTTTC
ACAACGCGATTGATGTAGN

Forward: AATGACTGGTATTGACCAAGGG
Reverse: CAACATATTCCCCGATTTGG
SSR motif:(TC)3 C (TC)16
Annealing temperature: 55-60°C
Observed alleles: 3
Expected size: 227 bp
Chromosome LDN substitution line and DT analysis: 7BL
Comments: Polymorphic between Messapia and MG4343: mapped on 7BL (close to the centromere, between XksuH1 and Xubp9).

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gpw93010
AATCCGCGGGCTTGAGAGCAAGAACACTAGAGAGAGAGAGAGAGAGAGAGAGCAATGGTG
ATTTTTTTCTGGAGGTAGGAGATGGAATGGGCTGAAGCGATAAGTGAGGAGGCCCACGTG
GTGACCACAACCCATCAGGGCGCCTGGGCTGCAGGCGTGCCCAGGTGGGTTGTGCCCACC
TGGTGCACCCCCCCTCTCATGTTATTTGCACTAAAAATTCAGAAATAGTCAAAAGAAACG
TATTAAATTTCTAGGGCATTTAGAGAACTTTTATTTTTGGGTCATTTTTTATTGCAAGGG
AAAATTCAAAAAAAGATAAAACATGGCATTTTAATTTATTTAACTAAGAAAAACAGAAAA
AGAAAGATAAGGACAGAAGGTAGTGCTCATTAAATTCATCAACTTCATACCCCTCAAAGA
TGATCCATTAATATGGTTGATCAAGTCTTATTAACAACCACCT

Forward: GGGCTTGAGAGCAAGAACAC
Reverse: GGCCTCCTCACTTATCGCTT
Reverse 2: GGCCTCCTCACTTATCGCTT
SSR motif:(AG)12
Annealing temperature: 60°C
Alleles observed: 1
Expected size: Fow-Rev 107, Fow Rev2 134 bp
Chromosome LDN substitution line analysis: Fow.-Rev. not essayed; Fow.-Rev.2 unlocated
Comments: Fow.-Rev. combination produces a complex banding pattern with several faint bands (250-900 bp). Fow.-Rev.2 (following photo) produces a monomorphic strong band (>400bp). Probable intron(s) between primer regions.

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gpw93013
AAAAGGCCATGATATGTCTCTATTGTATCTATAATTCTTGATTGTTTCATGCCAATATTC
TAGAACTTTTATATACATTTGGCGATATTTTATATTATTTTTGGGACTAACATATTGATC
CAGTGCCCAGTGCCAGTTCCTTTTTTTTGTTTCACAGAAAATCCATATCAAACGAAGTCC
ACACGCGATAAAATTTTACGAAGATTTATTTTGGAATATATGTGATTTTTGGGAAGAAGA
ATCAACAGAAGACAGTGCCCGAGGGACCCACAAGGCAATAGGGCACACCCTGGGGTAGGG
CGTGCCGTGTTTCATTGTGACTCCCTCATAAGTCGGTTGGAGCCCTTCTTTCGCCGCAAG
AAAGATAATTTCCATAGAATAATACCTACAAACTTTTAGCCCAATCAGAGTTACGGATCT
CCAGGAATAAAAAAAGTGGTGAAGGGATCCGAAAACAGACGTGAAACAAAAGAGGAACAG
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATCCAATCTCGGAGGGGC
TCGTGCCCTTCGGGAGCCATGGCGGCCAAGGAACAGAGATGAAACCCTCCTCCCATCTAG
GGGGAGTCCAAGGAAGAATAAGGAGGGGGGAGGGACTCTCCCCTCTCTTCCGGTGGCACC
AGAGCACCT

Forward: GTGAAGGGATCCGAAAACAG
Reverse: ATGGGAGGAGGGTTTCATCT
SSR motif:(AG)22
Annealing temperature: 60°C
Observed alleles: 5-6
Expected size: 158 bp
Chromosome LDN substitution line analysis: 1BL
Comments: Polymorphic between Messapia and MG4343: mapped on 1BL (between Glu-B1 and Xutv618)

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gpw93017
GCGAACAACATCAGCAGACTTGGAAGATTTTATCATAAGAAAAAAAGATGCTCGTGTTCC
TACTGTTTCAAAATTCTTCGCAATCGCCTGCAACAGCATAGGCGATCAGAAGAGGATCAC
GATATGGACTCTGCATGAGTTTGTGTTTGTTGAGAGAGTCGGATATTTTCCGATTAACTG
AAAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCCAGGAAAAAAGGCTTACGTGAGAGA
TATCTGGGAGGAGACACATTGGCCCTCCTTCTTCCTCGCCCTCCGGGTGTCTTGATTTCT
TCTTCTAACTCGTAGGCCGGGAGCAGTCCAAAATAGTTCGCCGTTGATGCAGCCGCCAGG
CAGTAGAGGGATTGGGGAATTGGATTCCATGTCAGCGCCATCGCTGATTTTACCAATTAC
TCGGTTAGAAAAACACACCTTCGTCCTTCTATAGAGC

Forward: ATTCTTCGCAATCGCCTG
Reverse: TCCCCAATCCCTCTACTGC
Reverse 2: AAGAAGGAGGGCCAATGTGT
SSR motif:(AG)15
Annealing temperature: 55°C
Observed alleles: 3
Expected size: 306
Chromosome LDN substitution line analysis: not locateded
Comments: Polimorphic between Messapia and MG4343: mapped on 6BS (completely linked to Gli-B2); the MG4343 allele is more than 100 bp bigger than the Messapia one.

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gpw93022
AGAAAACTTCAATGGGAAAACTCAATTCATCAAGAAGATAGAGAGGGGAAAAACTCCATA
TGATTCATCTATGTCAACAAAGACCGCGTTACATCAAGATCGTTCCATCTCAAGAACACG
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATACAACA
CATACCCTCGGCCCCGAGGGAGGACTACTCCCTCCTCATCATGGTGGCTGCCGGGATGAT
GAAGCTGTCCACCGGTGATGATCCCCCCCTCCGGCGGAGTGCCGGAGAAGGGCTCTAGAT
N

Forward: CAAAGACCGCGTTACATCAA
Reverse: CCGGTGGACAGCTTCATC
SSR motif:(GA)27
Annealing temperature: 60°C
Observed alleles: 1
Expected size: 178
Chromosome LDN substitution line analysis: Unlocated
Comments: Monomorphic in the 12 varieties screened.

 

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gpw93025
AAATCATGTTATGTTGATACAAAGCACAAAATATTTTGCGCAGATAAAAGGCAGGTTCGT
GGCAGAGCCGTAGAAGACGGCAATGTACGTAACTGCCGTATGGCGAGTGTTACAGAAGAT
CCTGCTGTGTAGGCGAGTGTGTGTGAGCCTGAGCCAGTGATCCCTCCCCCGGGTTTTCTC
CTTTTTTACATTCTCTTCTCCACTTTCCCTTTTGCCTTGTCAAAACCCTATAAATGGCGT
CCCAGTCCCCCCCCCCCAAACCCCCCGAATCCCACCTAGCTCATCCGCGCTTCTCTCTCT
CTCTCTCTCTCTCTCTCCCTCTCTCCCTCTCTACATCTACCTTGGATCCTCCAC

Forward: AGACGGCAATGTACGTAA
Reverse: AGGATCCAAGGTAGATGTAG
SSR motif:(TC)13 CC (TC)3
Annealing temperature: 60°C
Expected size: 276 bp
Observed alleles: 2
Chromosome LDN substitution line analysis: lower band 4A, higher band unlocated, but my LDN 4D(4B) could be not working.
Comments: monomorphic between Messapia and MG4343, not mapped.

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gpw94003
tgtcaactacgtacgtttgagcggcgagtaaccttcgtcacgccaaagagggtttgtgat
ttaagatttcaaatgaagccaacatggtcagttttgtgttttgctagattttactcgttg
tgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtctgtatctgcttggtacttaaagca
tgcgtgcccggaaatgtaggtaatgaccatggaggtttggcgcagcatacagtagaaagg
gcccgctttggtgatcctcgcatcttccacgaggtgcattacatgtgtgacagggagaaa
ggagtggtattggaggagagtgtagtgatggctccctaaacctcacggttcatgttcctc
attgcaaatttgcaattagagcatctatagcaagagacaacaaattcaggccctcaaacg
cccgcggacacgtctcatttgttggctaaccgcgtctctcatatacgaaacctcaaaact
attcaatccatgcaacgctacgtaaaa

Forward: AGTAACCTTCGTCACGCCAA
Reverse: CACTCTCCTCCAATACCACTCC
SSR motif:(TG)18
Annealing temperature: 60°C
Observed alleles: 5-6 (including nulli)
Expected size: 296 bp
Chromosome LDN substitution line analysis: higher band (the polimorphic one) 3B, lower band on 3A. No additional D band were observed.
Comments: Monomorphic between RIL parental lines.

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gpw95008
cgttgtgtatacatgagagaacggtcttctattttacttgtgtgtgtgtgtgtgagagag
agagagagggagcatccgtaacacaacaaccatctctcttgattcgtccgtccctcgtac
tatcgcatgtaacacatgcatcaacgcacacattttgacaccctcacccggcccctgccc
acctcaaggcccgcacatcgctctctatctcgcacgcacaatctcttcatgaaaacccta tttagcatgtagctttccgtgacatacacacacatttctctccataggtttgatttctct
caatatctgacacacacacacacaccacacacacaaaccccctcccctgagcacacaatt
catccccatccaaggttatatggcgaccactctcacttgtgcttctctgc

Forward: TAACACAACAACCATCTCTC
Forward 2: CATGAGAGAACGGTCTTCTATTTTAC
Reverse: GAGTGGTCGCCATATAAC
SSR motifs:(TG)8 (AG)7 and (AC)4… (AC)8 C (AC)4
Annealing temperature: 60°C
Observed alleles: 1
Expected size: Fow2-Rev 381 bp; Fow-Rev 314 bp
Chromosome NT analysis: unlocated
Comments: Very strong amplification with Fow2-Rev (following photo), but smaller sizes than expected (<200 bp). Strong background with Fow-Rev.

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gpw95014
atgtagaaaataacataggtaggtgaaaaaactgaaagcgtaaattcacagcaagaagcg
tattgtgacggtgaacccgacaaagtcaatccgtgctttattattagggatagactagga
aaagagccagtgcgttgcaacggaagaaaaacataacacacgctcttaacccaacaacca
tcactcaataggcccatcttttttatttttgcgaggcatcatatttgtgttgccgcttat
cctccttctcaccctcgccggcgatggcctcggtgttcacacaaaacaacaaaaaacgtg
tttgaatatggttaatcctaaggcgtctctctctccctctctcctccctcgccctctctc
tctctctctctcactttctctcactctcgcgatgagaaatctattgttttcccctgcgac
atttttcagaggtatgcatgtgtag

Forward: atttgtgttgccgcttatcc
Reverse: TGCATACCTCTGAAAAATGTCG
SSR motif:(TC)5 CC (TC)3… (TC)9
Annealing temperature: 60°C
Observed alleles:
Expected size: 216
Chromosome LDN substitution line analysis: 1A
Comments:.The amplified fragment is bigger than expected (>400 bp), probably because of intron presence on genomic DNA. Due to the size, it was difficult to read the electrophoretic pattern.

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Lines essayed:

1. T. aestivum (Chinese Spring)
2. T. turgidum durum (Langdon)
3. T. turgidum durum (Messapia)
4. T. dicoccoides (MG4343)
5. T. turgidum durum (Creso)
6. T. turgidum durum (Latino)
7. T. turgidum durum (Primadur)
8. T. turgidum dicoccum (MG5323)
9. T. turgidum dicoccoides (MG29896)
10. T. turgidum durum (Duilio)
11. T. turgidum durum (breeding line)
12. Triticale (Rhino)

In bold the parental lines of 65 RIL (Blanco et al. 1998: Theor Appl Genet 97:721-728).