AWN Vol 42

Release of EMBRAPA 40.

C.N.A. de Sousa, A.L. Barcellos, A.M. Prestes, E.N. Guarienti, E.P Gomes, J.C.S. Moreira, J.F. Sartori, L. de J.A. Del Duca, P.L. Scheeren, S.D. dos A. e Silva, and W.I. Linhares.

The wheat cultivar `EMBRAPA 40' was selected from the cross `PF 7650/NS 18-78//CNT 8/PF 7577' made in Mexico in 1979 and bulked in the F6 generation as a line (PF 84316) in 1984 using the mass method. EMBRAPA 40 was released for cultivation in the state of Rio Grande do Sul in 1995. This variety yielded 2,812 kg/ha, 1 % over the average of the check cultivars according to the average of 66 trials conducted in Rio Grande do Sul from 1992-94. EMBRAPA 40 is an early, tall spring wheat with some tendency to lodge. This cultivar is susceptible to powdery mildew, but resistant to all races of Puccinia graminis tritici found in Brazil. EMBRAPA 40 is susceptible to some races of P. recondita, but, so far, has good field resistance to leaf rust and is moderately resistant to soilborne mosaic virus. This cultivar has good resistance to sprouting and moderate tolerance to aluminum toxicity. The industrial quality of EMBRAPA 40 is considered good, and it has the high molecular weight glutenin subunits 5+10 and 17+18.

1995 Wheat Cultivar Yield Trials in Passo Fundo, Brazil.

J.C.S. Moreira and C.N.A. de Sousa.

About 350 wheat genotypes were tested in 19 yield trials at the National Research Center for Wheat of EMBRAPA in Passo Fundo, Rio Grande do Sul, Brazil, during 1995. The process for releasing a new cultivar in Rio Grande do Sul, the southernmost Brazilian state, was described in the 1986 Annual Wheat Newsletter (32:38-39).

The climatic conditions in Passo Fundo during the wheat growing season (June to November) were not good for wheat. A drought lasted through much of the growing season. Plants had a poor growth and the yield was low. Disease was less than in previous years.

The field trials were in a rotation area (2 years without wheat) with fertilizer applications of 12.5 kg/ha N, 63 kg/ha P205, 50 kg/ha P205, 50 kg/ha K2O, and 45 kg/ha N as top-dressing. The trials were conducted in a no-till system. No fungicides were used in most trials.

The cultivars used as controls in 1995 were BR 23, CEP 24, and EMBRAPA 16. The cultivar EMBRAPA 16 is the main cultivar grown in Rio Grande do Sul, occupying about 100,000 ha (30 %) of the growing area in this state. For several years BR 23 was the main cultivar grown in this region of Brazil.

No entry exceeded the yield of the three local checks in the South Central Brazilian Trial. Cultivars that yielded more than EMBRAPA 16 in trials in Passo Fundo, RS, are listed in Table 2.

Table 2. Cultivars with higher yield than EMBRAPA 16 in trials in Passo Fundo, RS, in 1995.

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Yield % of

Cultivar Pedigree kg/ha EMBRAPA 16

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1. Cultivar State Trialówithout fungicide.

BR 23 CC/ALD SIB/3/IA5 54-20/COP//CNT 8 2,743 106

BR 35 IAC 5*2/3/CNT 783/LD//IAC 5/Hadden 2,721 106

RS 1-Fenix PF 70100/J 15157-69 2,701 105

2. Cultivar State Trialówith fungicide.

BR 23 CC/ALD SIB/3/IA5 54-20/COP//CNT 8 3,046 111

BR 35 IAC 5*2/3/CNT 783/LD//IAC 5/Hadden 3,173 115

RS 1-Fenix PF 70100/J 15157-69 2,780 101

3. South Brazilian Trialówithout fungicide.

PF 9099 PF 82252/BR 35//IA 7998/PF 8550 2,667 103

BR 23 CC/ALD SIB/3/IA5 54-20/COP//CNT 8 2,661 103

4. South Brazilian Trialówith fungicide.

CEP 9168 CEP 8435/CEP 17//BR 23 2,723 102

ORL 91256 BR23/PF 869107 S 2,738 103

BR 23 CC/ALD SIB/3/IA5 54-20/COP//CNT 8 2,991 112

Table 2 (Cont). Cultivars with higher yield than EMBRAPA 16 in trials in Passo Fundo, RS,in 1995.

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Yield % of

Cultivar Pedigree kg/ha EMBRAPA 16

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5. Regional Yield Trial.

SA 9252 RS 4/2* Minuano 82 2,651 104

SA 9458 PF 82250/RS 1 2,630 103

BR 23 CC/ALD SIB/3/IA5 54-20/COP//CNT 8 2,991 112

6. Multilocation Preliminary Trials (5 sites with lines in 2nd year of test). Data from

Passo Fundo.

PF 92140 BR 35/PF 85946/3/PF 772003*2/PF 813//PF 83899

2,670 106

PF 92141 BR 35/PF 85946/3/PF 772003*2/PF 813//PF 33899

2,554 101

PF 92231 CEP 14/BR 23//CEP 19 2,609 103

PF 92392 BR 35*3//BR 14*2/Largo 2,592 103

PF 92398 BR 35*4/3/PF 839100//BR 14*2/CI 17959 2,595 103

PF 92569 BR 35*2/PF 85437 2,844 113

PF 92570 BR 35*2/PF 85437 2,632 104

7. Preliminary trials (1st year trial - 11 preliminary lines, 235 lines).

5th Preliminary trial.

PF 940097 PF 8619/BR 35 2,346 101

PF 940099 PF 8619/BR 35 2,857 101

PF 940110 PF 83743/PF 813019//PF 84296/PF 83743 3,122 111

7th Preliminary trial.

PF 940156 BR 34/PF 8545 2,123 102

PF 940159 BR 35/PF 8596/3/PF 772003*2/PF 813//PF 8399 2,256 103

PF 940176 PF 8569/3/VS 73-98//PF 802014//PF 802014/F 29-76

2,186 105

PF 940181 BR 35*2/Coker 80-33 2,207 106

PF 940182 PF 87511*2/Coker 80-33 2,318 111

PF 940184 PF 87511*2/Coker 80-33 2,218 106

8th Preliminary trial.

PF 940200 PF 87511*2/Coker 80-33 2,736 121

PF 940201 PF 87511*2/Coker 80-33 2,844 126

PF 940202 PF 87511*2/Coker 80-33 2,622 116

PF 940207 BR 35/TP 2,549 113

PF 940212 Coker 762/BR 23//Coker 762/BR 14 2,707 120

9th Preliminary trial.

PF 940266 BR 23//CEP 19//PF 35490 2,574 102

8. High Technology Trial (with fungicide and a high amount of nitrogen).

ORL 9128 PF 869107 S/BAU S 3,169 120

PF 86242 HLN/CNT 7//Amigo/CNT 7 2,850 108

SA 9458 PF 82250/RS 1 2,950 112

BR 23 CC/ALD SIB/3/IAS 54-20/COP//CNT 8 2,709 103

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Observations on cultivar resistance to scab.

C.N.A. de Sousa.

Scab (Fusarium graminearum, syn. Gibberella zeae) was an important disease in the crossing block conducted in the field in Passo Fundo, Rio Grande do Sul, Brazil, for the last 3 years. A survey through simple observation was done, although it is not an easy task to take notes on this disease. The best lines (cross in parentheses) of those lines observed for 3 years in the crossing block were Ning 8331, PF 84511 (Butui/PAT 73g2), PF 859114 (CNT 10//LD*3/NBAY), PF 89156 (Sulivan/PF 79777), PF 9099 (PF 82252/BR 35//IA 7998/PF 8550), PF 90132 (VS 73-598/BR 6), Toropi, Trigo BR 32, and Wuhan 3. Era, GD 88158, Oasis, PF 9052 (PF 8237//LAP 689/3*CNT 10), and PF 83145 (CEP 14/PF 79547) were the best of the lines tested for 2 years.

A survey for the presence of Ne genes.

C.N.A. de Sousa.

In 1995, a survey was made to determine the presence of the Ne1 and Ne2 genes in several Brazilian cultivars. Together, these genes promote hybrid necrosis. Simple crosses were made using genotypes with the Nel or Ne2 genes and the cultivar to be tested. When crosses between a cultivar with Ne1 and another with Ne2 are made, hybrid necrosis occurs in the F1 generation. The main reason for this survey is that the Ne2 gene is located near the Lr13 gene for adult-plant leaf rust resistance. Lr13 seems to be important in combination with other genes for leaf rust resistance. Brazilian cultivars EMBRAPA 16, PF 9099, Trigo BR 18, Trigo BR 35, Trigo BR 43, and C306 (BRA) exhibited hybrid necrosis in the F1 when crossed with Ne1 lines, indicating the presence of the Ne2 gene and, by extension, gene Lr13. PF 844003 and PF 89419 have the Ne1 gene, which is a rare trait among Brazilian wheats. Cultivars CEP 27, Colonias, IAPAR 17, OCEPAR 10, PF 88305, PF 89230, PF 92432, PF 9323, and Trigo BR 42 do not have either Nel or Ne2. CEP 24, EMBRAPA 27, EMBRAPA 40, PF 79547, PF 9132, PF 9210, PF 9234, PF 92349, PF 93113, PF 9325, Trigo BR 20, and Trigo BR 36 do not have the Ne2 gene and were not tested for Ne1.

Wheat soilborne mosaic virus.

A.M. Prestes, P.L. Scheeren, C.N.A. de Sousa, M. Barbosa, and L.R. Goulart.

The reaction of more than 300 entries of wheat, comprising a crossing block and final wheat yield trials, were evaluated in 1994 and 1995 for resistance to SBMV under field conditions at Passo Fundo, in the state of Rio Grande do Sul, Brazil. The disease is endemic in the northern part of the state and may cause severe losses to susceptible cultivars.

A nursery was established in a field where SBMV was severe in previous years. All cultivars were sown in double rows, 2 m long, after a rain when soil was still very wet. Evaluation was based on symptomatology using a 0(zero) to 5 scale considering the severity of symptoms, stunting, and rosette formation.

Considering the 2-year evaluations, a few cultivars had good resistance to SBMV (Table 3). EMBRAPA 16, the widest grown commercial variety in the southern state, was the most resistant cultivar. EMBRAPA 15, ENBRAPA 40, BR 32, and BR 38 were moderately resistant. Among the foreign germplasm, Amigo and Century were the most resistant, whereas Coker 762, Coker 80-33, Coker 97-33, Florida 301, Hadden, Hunter, Era, Newganes, Oasis, and Tivoli were susceptible.

Table 3. A few examples of soilborne mosaic virus-resistant cultivars from the 1994-95

nursery.

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Cultivar Pedigree Score

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Amigo Gaucho/TCS/63PC42-4/3/Teewon (BC)/4/... 0

Century Tanya/TAM W101/Amigo 0

EMBRAPA 15 CNT 10/BR 35//PF 75172/Tifton 2

EMBRAPA 16 HLN/CNT 7//Amigo/CNT 7 0

EMBRAPA 40 PF 7650/NS 18-78//CNT 8/PF 7577 2

Frontana Fronteira/Mentana 2

TRIGO BR 4 IAS 20*3/Sinvalocho Gama 1

TRIGO BR 8 IAS 20/TP//PF 70100 2

TRIGO BR 32 IAS 60/Indus//IAS 62/3/ALD SIB/4/IAS 59 2

TRIGO BR 36 JUP 73*3/Amigo 1

Toropi (check) PE 8//FN 1971-37/QNA-A 5

Florida 301 (check) Holley/3/Olesen//Arthur 71 5

Coker 762 (check) Unknown 4

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The resistance of EMBRAPA 16 and of a number of other cultivars may come from Amigo. Studies involving reciprocal crosses of EMBRAPA 16 with the susceptible cultivars BR 23, BR 35, and EMBRAPA 27 indicated that the resistance in these crosses was governed by two dominant genes with interacting effects. Additional studies to determine the inheritance of resistance to SBMV are in progress.