IV. Cultivars and Germplasm

IV. CULTIVARS AND GERMPLASM


USDA-ARS NATIONAL SMALL GRAINS GERMPLASM RESEARCH FACILITY

P.O. Box 307, Aberdeen, ID 83210, USA.

National Small Grains Collection wheat germplasm evaluations.

H.E. Bockelman, D.M. Wesenberg, C.A. Erickson, B.J. Goates, and S. Nieto, National Small Grains Germplasm Research Facility, USDA­ARS, University of Idaho Coöperating, Aberdeen, Idaho.

The USDA-ARS National Small Grains Collection (NSGC) is one of the several components of the National Plant Germplasm System. The NSGC is a working collection in contrast to the base collection at the National Seed Storage Laboratory (NSSL) at Fort Collins, Colorado. The numbers of accessions in the NSGC are summarized in Table 1.



Table 1. Number of accessions per genus in the USDA-ARS National Small Grains Collection, February, 1998.

Taxonomy NSGC Accessions
Triticum 46,036
Hordeum 27,332
Avena 21,252
Oryza 17,595
Aegilops 2,157
Secale 1,914
X Triticosecale 1,381
All Species 117,704

 

The systematic evaluation of wheat accessions in the NSGC and other elite germplasm continued to be coordinated or conducted by National Small Grains Germplasm Research Facility (NSGGRF) staff at Aberdeen during 1997. Maintenance and evaluation of NSGC small grains germplasm, including quarantine entries, also continued at Maricopa, Arizona in 1996-97 under the supervision of S. Nieto. Cooperative NSGC wheat evaluations continued for reaction to Hessian fly; BYDV; stripe, leaf, and stem rust; powdery mildew; and dwarf bunt, as well as ploidy analysis of Triticum species. Evaluation for RWA reaction is essentially complete. The Aberdeen staff has been involved directly in the entry of NSGC evaluation data into the GRIN system and the evaluation of growth habit of NSGC wheat accessions. Under the direction of H.E. Bockelman, the NSGC staff distributed more than 21,000 NSGC accessions in 1996 and more than 25,000 in 1997 to scientists in the U.S. and worldwide.

Specific cooperative agreements or fund transfers within ARS involving coöperative evaluations and related research for all small grains involve several University and ARS projects in at least 15 states. Fund transfers concerned with wheat germplasm evaluations involve Pullman, WA (Line), West Lafayette, IN (Ratcliffe), Manhattan, KS (Hatchett and Eversmeyer), Davis, CA (Qualset), and Stillwater, OK (Webster).

A specific cooperative agreement entitled 'Evaluation of Wheat Germplasm for Resistance to Karnal Bunt Disease' was initiated in 1996 with CIMMYT. Dr. Jesse Dubin, Dr. G. Fuentes Davila, and Dr. S. Rajaram are the CIMMYT investigators on the project. The project is designed to evaluate the reaction of established spring wheat varieties, elite spring wheat germplasm, and selected NSGC accessions to Karnal bunt disease. Initial evaluations involved spring and facultative wheat. A 200-entry 1996-97 Karnal Bunt Evaluation Trial, composed of 199 spring and facultative wheat varieties and selections and Betzes barley was planted in Mexico for evaluation of resistance to Karnal bunt disease. A similar nursery composed of 300 entries is being evaluated in the 1997-98 season by CIMMYT. Research is being performed at CIMMYT facilities at Ciudad Obregon, Sonora, Mexico. The wheats include proprietary varieties developed by AgriPro, Arizona Plant Breeders, Northrup King, Pioneer HiBred International, Inc., Resources Seeds, Inc., Western Plant Breeders, and World Wide Wheat (Seeds West), as well as cultivars and selections developed in the public sector in California, Florida, Georgia, Idaho, Louisiana, Montana, North Dakota, Oregon, South Carolina, South Dakota, Texas, Utah, Washington, California/Australia, CIMMYT/California, Pioneer/North Dakota Research Foundation, USDA-ARS/Idaho, USDA-ARS/Minnesota, Washington/Idaho/USDA-ARS, and Washington/Oregon. Market classes currently being considered include durum, HRSW, SWSW, and HWSW. A summary of Karnal bunt evaluation data for the 1996-97 Karnal Bunt Evaluation Trial was distributed to cooperators. Additional copies of the summary report are available upon request.

Descriptors appropriate for wheat have been established in collaboration with the Wheat Crop Germplasm Committee. Field evaluation data are recorded on such descriptors as growth habit, number of days from planting to anthesis (heading), plant height, spike or panicle density, lodging, straw breakage, shattering, and awn and glume characteristics, including color. Special nurseries are grown for that purpose at Aberdeen, Idaho, and Maricopa, Arizona.

Data obtained from evaluations of NSGC germplasm are entered in the Germplasm Resources Information Network (GRIN) system by the NSGGRF staff in coöperation with the ARS National Germplasm Resources Laboratory, Beltsville, Maryland. GRIN is a database containing the characteristics and availability of all genetic resources included in the National Plant Germplasm System. The Database Manager is J.D. Mowder, Beltsville, Maryland. The NSGGRF staff interacts with the GRIN system in recording NSGC orders (seed requests), entering a variety of data, and conducting information searches. No evaluations have been conducted to date for descriptors such as drought tolerance, salt tolerance, winter hardiness, Cephalosporium stripe, flag smut, leaf blight, loose smut, snow mold, take-all, tan spot, WSMV, and protein.

Triticum descriptors with data currently on the GRIN system are summarized in Table 2.

 

Table 2. National Small Grains Collection evaluation of disease; insect; and agronomic, taxonomic, and quality data for wheat on the GRIN system (updated February, 1998).

 Character  Years  Location  Number of accessions
 Disease evaluations.
 Barley yellow dwarf virus  1985-92  Davis, CA  2,287
 Barley yellow dwarf virus  1988-94  Urbana, IL  17,517
 Soilborne mosaic virus  1985-89  Urbana, IL  6,587
 Leaf rust  1983-89, 91-95  Manhattan, KS  38,753
 Stripe rust - adult  1984-95  Mt. Vernon, WA  30,523
 Stripe rust - adult  1984-95  Pullman, WA  21,800
 Stripe rust - Cdl 17  1984-95  Pullman, WA  15,455
 Stripe rust - Cdl 20  1984-95  Pullman, WA  12,508
 Stripe rust - Cdl 25  1984-95  Pullman, WA  1,682
 Stripe rust - Cdl 27  1984-95  Pullman, WA  14,511
 Stripe rust - Cdl 29  1984-95  Pullman, WA  14,259
 Stripe rust - Cdl 37  1984-95  Pullman, WA  1,851
 Stripe rust - Cdl 43  1984-95  Pullman, WA  1,805
 Stripe rust - Cdl 45  1984-95  Pullman, WA  1,880
 Stem rust - adult  1987-94  Rosemount, MN  8,078
 Stem rust - adult  1987-94  St. Paul, MN  19,141
 Stem rust - HJCS  1987-92  St. Paul, MN  4,342
 Stem rust - QFBS  1987-92  St. Paul, MN  8,639
 Stem rust - QSHS  1987-92  St. Paul, MN  4,455
 Stem rust - RHRS  1987-92  St. Paul, MN  4,312
 Stem rust - RTQQ  1987-92  St. Paul, MN  8,973
 Stem rust - TNMH  1987-92  St. Paul, MN  4,402
 Stem rust - TNMK  1987-92  St. Paul, MN  8,938
 Stem rust - HNLQ  1987-92  St. Paul, MN  4,705
 Stem rust - RKQS  1987-92  St. Paul, MN  4,682
 Stem rust - genes  1987-92  St. Paul, MN  1,018
 Common bunt - R36  1981-92  Aberdeen, ID *  74
 Common bunt - R39  1981-92  Aberdeen, ID *  1,422
 Common bunt ­ R43  1981-92  Aberdeen, ID *  318
 Common bunt ­ T­1  1981-92  Aberdeen, ID *  6,301
 Common bunt ­ multiple  1981-97  Aberdeen, ID *  10,933
 Dwarf bunt  1978­97  Aberdeen, ID **  10,456
 Septoria nodorum  1970­78  Bozeman, MT  8,095
 Insect evaluations.
 Hessian fly - B  1983-94  W. Lafayette, IN and Manhattan, KS  449
 Hessian fly - C  1983-94  W. Lafayette, IN and Manhattan, KS  24,165
 Hessian fly - E  1983-94  W. Lafayette, IN and Manhattan, KS  24,149
 Hessian fly - GP  1983-94  W. Lafayette, IN and Manhattan, KS  14,441
 Hessian fly - L  1983-97  W. Lafayette, IN and Manhattan, KS  8,315
 Russian wheat aphid  1988-95  Stillwater, OK  40,841
 Cereal leaf beetle  1963-70  Indiana and Michigan  16,347
 Agronomic, taxonomic, and quality evaluations.
 Growth habit  1987-97  Aberdeen, ID  41,768
 Chromosome number  1988-91  Columbia, MO  519
 Market class      975
 Lysine content  1966-69  Lincoln, NE  10,367
 Awn color  1983-97  Aberdeen, ID and Maricopa, AZ  22,652
 Awn type  1983-97  Aberdeen, ID and Maricopa, AZ  26,563
 Glume color  1983-97  Aberdeen, ID and Maricopa, AZ  22,814
 Glume pubescence  1983-97  Aberdeen, ID and Maricopa, AZ  24,314
 Heading date  1983-94  Aberdeen, ID and Maricopa, AZ  18,365
 Kernel color  1983-94  Aberdeen, ID and Maricopa, AZ  21,319
 Kernels / spike  1983-94  Aberdeen, ID and Maricopa, AZ  3,666
 Kernel weight  1983-94  Aberdeen, ID and Maricopa, AZ  3,669
 Leaf pubescence  1983-94  Aberdeen, ID and Maricopa, AZ  20,890
 Plant height  1983-97  Aberdeen, ID and Maricopa, AZ  21,841
 Rachis length  1995  Maricopa, AZ  2,512
 Shattering  1983-94  Aberdeen, ID and Maricopa, AZ  10,637
 Spike density  1983-97  Aberdeen, ID and Maricopa, AZ  13,681
 Spikelets / spike  1995  Maricopa, AZ  2,502
 Spike type  1983-97  Aberdeen, ID and Maricopa, AZ  15,553
 Straw breakage  1983-94  Aberdeen, ID and Maricopa, AZ  16,831
 Straw color  1983-97  Aberdeen, ID and Maricopa, AZ  19,624
 Straw lodging  1983-94  Aberdeen, ID and Maricopa, AZ  23,077
 * 1985-86 Pendleton, OR; ** field tests are conducted at Logan, UT, by Aberdeen ARS staff.

 

Similar evaluations are currently underway for other major NSGC components, including barley, oats, rice, and triticale. Other important cooperative projects, especially involving wheat, include the Specific Cooperative Agreement Acquisition, Evaluation, and Conservation Strategies for Small Grains Genetic resources (University of California, Davis - C.O. Qualset). B.J. Goates annually conducts evaluations of wheat germplasm for bunt resistance at Aberdeen, Idaho and Logan, Utah.

The authors wish to acknowledge the important contributions of the NSGGRF staff in this effort, with special thanks to Glenda B. Rutger, Scott McNeil, Judy Bradley, Carol S. Truman, Kathy E. Burrup, Dave E. Burrup, Kay B. Calzada, and Mark A. Bohning.


Wheat genetic stocks collection.

An Hang.

A major portion of the E.R. Sears Wheat Genetic Stocks Collection has been transferred to the USDA-ARS National Small Grains Germplasm Research Facility at Aberdeen, Idaho. These stocks consist of various aneuploids of Chinese Spring wheat, including: monosomic, trisomic, tetrasomic, nullisomic-tetrasomic, ditelosomic, double monotelosomic, dimonotelosomic, monotelodisomic, double ditelosomic, and various addition, substitution, and translocation lines. A list of these stocks and seed are available upon request to Dr. An Hang, USDA-ARS, P.O. Box 307, Aberdeen, ID 83210.


National Small Grains Collection activities.

H.E. Bockelman.

Cultivar name clearance. Breeders in the United States are encouraged to have proposed names for new cultivars checked for duplication, trademark, and other possible infringements. The National Small Grains Collection will be glad to assist you. Send the proposed name to Harold E. Bockelman, USDA-ARS-NSGC, P.O. Box 307, Aberdeen, ID 83210, Fax 208-397-4165, E-mail nsgchb@ars-grin.gov. If desired, more than one name can be submitted, listed in order of preference. This will save considerable time if a conflict is found with the first name. Available records (GRIN, CI/PI cards, and variety files) here at Aberdeen are checked for conflicts with the proposed name. If a conflict is found (previous use of the name for that crop), the breeder is requested to submit a different name. If no conflicts are found, the requested name is forwarded to the Agricultural Marketing Service where the proposed name is checked for possible conflicts in trademarks The Agricultural Marketing Service does not guarantee that its finding is the final word, because their files may not be complete and/or unregistered trademarks may exist. This clearance procedure generally requires about 4 weeks.

Elite germplasm requested. Breeders are encouraged to consider submitting their elite lines for inclusion in the National Small Grains Collection. Of special interest are lines that have been in uniform nurseries but are not to be released as cultivars. Historically, uniform nurseries been the testing grounds for the most advanced, elite germplasm from the various public and private breeding programs. Entries in uniform nurseries and other breeding materials that are never released as cultivars are still of potential value to breeders, pathologists, entomologists, and other researchers. Breeders should submit 200-500 g of untreated seed to the NSGC (address P.O. Box 307, Aberdeen, ID 83210). Seed from outside of the United States should be sent to the USDA Plant Germplasm Quarantine Center (address Bldg. 580, BARC-East, Beltsville, MD 20705) with enclosed forwarding directions. Provide a description of the germplasm, including: donor (breeder, institution); botanical and common name; cultivar name and/or other identifiers (breeder line or selection number); pedigree; descriptive information (of important traits and special characteristics); and growth habit. Assignment of a PI number and inclusion in the NSGC makes the germplasm available for research purposes to bona fide scientists in the U.S. and worldwide. Please note that a different procedure applies if you are obtaining Crop Science registration. Follow directions provided by the crop registration committee.

Guidelines for exporting seed. All seed sent to a foreign country should be inspected and receive a phytosanitary certificate. In most cases, a fee payable to APHIS (Animal & Plant Health Inspection Service) is required to cover the cost. You may wish to work with APHIS personnel in your state or your State Department of Agriculture to obtain a phytosanitary certificate. Please also be aware of any import permits and additional declarations that certain importing countries may require to accompany the shipment.

Guidelines for importing seed. Any scientist importing seed should be aware of any restrictions that apply. APHIS personnel can provide current information on applicable restrictions. Of particular importance to wheat researchers are import restrictions related to flag smut and karnal bunt. Presently, some 34 countries have flag smut import restrictions. Six countries currently have Karnal bunt import restrictions.

Importation of seed from flag smut and Karnal bunt countries requires a permit from APHIS. Special handling and grow-out procedures apply to such shipments.

PI Assignments in Triticum from January 1997-February 1998.

PI Assignments in Aegilops from January 1997-February 1998.

PI Assignments in X Triticosecale from January 1997­February 1998.