Items from Spain.
ITEMS FROM SPAIN
UNIVERSIDAD POLITECNICA DE MADRID
Departamento de Biotecnologia, E.T.S. Ing. Agronomos.- C. Universitaria,
28040, Madrid, Spain.
A. Delibes, I. López-Braña, M. J. Montes, and
C. González-Belinchón.
UNIVERSITY OF LLEIDA AND
INSTITUTE FOR FOOD AND AGRICULTURAL RESEARCH AND TECHNOLOGY (UdL-IRTA)
Center of R&D, Rovira Roure 177, 25198 Lleida, Spain.
J.A. Martín-Sánchez, E. Sin, C. Martínez,
and A. Michelena.
JUNTA DE EXTREMADURA. SERVICIO DE INVESTIGACION AGRARIA.
Departamento de Fitopatología. Ap. 22 CP. 06080 Badajoz,
Spain.
J. del Moral, F. Pérez-Rojas, F. J. Espinal, and M.
Senero.
Studies in relation to the Hessian fly-resistance gene
(H30) transferred from the wild grass Aegilops triuncialis to
hexaploid wheat. [p. 142-144]
The transfer line TR-3531 (42 chromosomes), derived from the
cross 'T. turgidum/Ae. triuncialis//T. aestivum'
and carrying the H. avenae resistance gene Cre7
(Romero et al. 1998), showed a high level of resistance to the
M. destructor biotype prevailing in southwestern Spain.
A single, dominant gene (H30) determines the Hessian fly
resistance in this introgression line (Delibes et al. 2001), and
its linkage with an isozyme marker (Acph-U1) has been studied.
A phosphatase marker, resolved into two components, is present
in the TR-3531 line, Ae. triuncialis (UC), Ae. umbellulata
(U), and the amphiploid Chinese Spring/Ae. umbellulata
(ABDU), but is absent in Ae. caudata (C). Linkage between
H30 and the Acph-U1 marker (associated with the
U genome) was determined by analyzing 126 individual (TR-3531/H-10-15)
F2 plants. The kernels were cut transversely and the halves without
embryos were used to obtain phosphatase zymograms, an enzymatic
system associated in wheat with homoeologous group 4 (Delibes
et al. 1997a). The linkage in this cross is not very tight, which
would be consistent with the recombination expected of the ability
of the C genome to suppress the Ph-diploidization mechanism
of wheat (Romero et al. 1998).
F2 progeny, derived from crosses between different wheat cultivars
from Uniform Hessian Fly Nursery (UHFN and H-93-33 transfer line)
and with other sources of resistance, and TR-3531 were tested
for resistance in field conditions in order to determine if the
new resistance gene was allelic with the H3, H5,
H6, H12, H13, H18, H21, or
H27 genes. Although the cultivars from UHFN are effective
against Hessian fly in the United States, there is no evidence
that the selected genes confer resistance to biotype present in
Azuaga (southwestern Spain). The results and summarized in Table
1. All UHFN cultivars tested with different genes were resistant
to this biotype, except the cultivar Abe with the gene H5,
which showed a inconsistent reaction. The resistance gene H30
in line TR-3531 is nonallelic with respect to the genes H3,
H6, H12, H13, H18, and H21
present in wheat cultivars from UHFN and H27 in the introgression
line H-93-33 (Delibes et al. 1997a and b). Previously, we demonstrated
that H30 in TR-3531 line was not allelic with respect H9
and H11 present in cultivars Ella and Kay, respectively
(Delibes et al. 2001).
Table 1. Hessian fly reactions of the parents,
F1, and F2 populations from crosses between wheats with different
resistance genes and the resistant line TR-3531, a carrier of
the H30 gene. Hessian fly reaction of line R-3531 is
25R:0S. R = resistance and S = susceptibility to Hessian fly.
UHFN = Uniform Hessian Fly Nursery.
Cultivar
or UHFN line |
Gene |
Chromosome |
Hessian fly reaction
Crosses between cultivars of the UHFN / TR-3531
|
UHFN
line |
No.
F1 plants |
No.
F2 plants |
X2(1:d.f.)
15:1 ratio |
R:S |
R:S |
R:S |
Value |
Probability
(P) |
Howell |
H3 |
5A |
18:0 |
7:0 |
154:8 |
0.46 |
0.5 |
Monon |
H3 |
5A |
19:0 |
9:0 |
187:6 |
3.25 |
0.05<P<0.1 |
Caldwell |
H6 |
5A |
29:0 |
8:1 |
218:14 |
0.02 |
0.9 |
841453 H15-1-1-2-5-2 |
H12 |
5A |
26:1 |
2:0 |
95:1 |
4.45 |
0.01<P<0.05 |
86925 RA1-16 |
H13 |
6DL |
18:2 |
3:0 |
140:7 |
0.55 |
0.3<P<0.5 |
Brule |
H18 |
--- |
15:4 |
7:0 |
141:2 |
5.74 |
0.01<P<0.05 |
KS86HF012-23-6 |
H21 |
2BS |
19:0 |
7:0 |
243:6 |
6.27 |
0.01<P<0.05 |
H-93-33 |
H27 |
4M^v^ |
21:0 |
7:0 |
50:5 |
0.76 |
0.3<P<0.5 |
Advanced lines with the H30 gene were obtained by backcrossing
the transfer line and different commercial wheats (cultivars Anza,
Betres, Cajeme, Cartaya, Marius, Rinconada, and Osona) as recurrent
parents. In all advanced lines, the infestation level was higher,
but in the same range, than the donor. Several agronomic characteristics
were studied in 16 advanced lines and the results of three of
the lines are summarized in the Table 2. The best results were
achieved with the Ma-6 line, which displayed good agronomic characteristics,
in comparison to the susceptible controls, for the three traits
studied. Another fact that increases the importance of this line
is that it also carries the CCN resistance gene Cre7.
Table 2. Agronomic characteristics of three
advanced lines with the H30 resistance gene in comparison
to three bread wheat cultivars.
Cultivar or line |
Yield (g/m^2^) |
Kernels/spikelet
|
1,000-kernel weight
(g) |
T. aestivum cv. Osona |
1,240.57 |
2.71 |
29.49 |
T. aestivum cv. Astral |
897.55 |
1.64 |
30.17 |
T. aestivum cv. Adalid |
1,687.51 |
2.65 |
33.14 |
Ma6: TR/OS//OS/3/RN/4/OS/5/RN/6/AZ 3 |
1,914.12 |
3.00 |
43.37 |
Ma4: TR/BT//AL/3/MA/4/3*BT 2 |
1,336.08 |
2.84 |
27.65 |
Ma3: TR/3*OS//4*CYÄ/3/CJ 4 |
1,486.86 |
2.64 |
32.50 |
Least significant difference (LSDP<0.05) |
409.16 |
0.47 |
5.05 |
Abbreviations used: Ma = advanced lines,
TR = TR-353 line, AZ = Anza, BT = Betres, CJ = Cajeme, CY = Cartaya,
MA = Marius, OS = Osona, RN = Rinconada, = selfing. |
Cooperation with other institutions. We are cooperating
with Acorex (Cooperative of Extremadura farmers).
Financial support. This work was supported by grants
AGL2001-3824-CO4, PTR 95-0496-OP CO1021001 from CICYT "Comision
Interministerial de Ciencia y Tecnologia" of Spain and IPR99A042
(Junta de Extremadura).
References.
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Mejías A, Gallego M, Casado D, Sin E and López-Braña
I. 1997a. Hessian fly-resistance gene transferred from chromosome
4Mv of Aegilops ventricosa to Triticum aestivum.
Theor Appl Genet 94:858-864.
- Delibes A, López-Braña I, Martín-Sanchez
JA, Sin E, Martínez C, Michelena A, Del Moral J and Mejías
A. 1997b. Transfer of one gene for resistance to Hessian fly
(Mayetiola destructor) from Aegilops ventricosa
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