A Database for Triticeae and Avena
II. 5. Wild barley (Hordeum L.) collected by Agriculture Canada
in Turkey, Iran and Greece.
I. L. Craig and G. Fedak, Ottawa Research Station, Agriculture Canada,
Ottawa, Ontario K1A OC6, Canada.
The Research Branch, Agriculture Canada, recognizing the importance
of collecting and conserving cultivated and wild species of barley organized
a collecting expedition in April and May 1978 when Dr. B. R. Baum, George
Fedak, and J. W. Martens conducted an extensive exploration of Turkey,
Iran and Greece.
The areas and specific sites of the collection was selected to represent
a wide range of diversity of habitat for each species reinforced by data
from the literature, local botanists and agronomists as well as from the
collectors' experience and knowledge of the species.
On their return to Canada, the seed data was collated and computerized
while the samples themselves were in a cold dormancy period. After dormancy,
four to eight seeds per accession were minutely dehulled. Each seed was
dipped in a 7% sodium hypochlorite and placed on filter paper in plastic
petri dishes. The filter paper was maintained in a dampened state for the
first two to three days, or until the radicle appeared, with 550 ppm GA3
in tap water and from then on only tap water. Somatic chromosome counts
were obtained using the standard Feulgen technique. Seedlings were planted
in soil and at the 2nd leaf stage were vernalized for ten weeks prior to
establishing a greenhouse nursery of from two to four plants from each
Table 1 shows the results of the chromosome survey to date.
Table 1. PLOIDY LEVEL
While the collection has a large number of accessions of H. vulgare
and H. spontaneum these species were not included in this study.
H. leporinum was the only hexaploid species. H. bulbosum
collected from 371 geographic sites was the largest species in the collection.
At least 1200 chromosome verifications were made and only tetraploids were
Specimens of all entries of species as well as future hybrids or polyploids
produced will be collected. Seed samples from each accession will be deposited
in the Plant Gene Resources of Canada. Morphological, fertility (cross-
or self-compatibility), annual or perennial growth data are being collated.
This first phase has been primarily of a survey nature; the second phase
now underway will evaluate the potential genetic resources which might
be incorporated into present crop varieties. Detailed chromosome analysis
will include karyotype studies of the somatic chromosomes thereby augmenting
studies by Rajhathy et al. (1963). Extensive crossing of all combinations
among and between species as well as with standard cultivars will employ
the culturing techniques of Fedak (1979) for barley x rye hybrids. The
somatic chromosomes data coupled with chromosome pairing studies will facilitate
accurate identification of species, the assessment of species relationships
within the genus and a rational crossing program.
Fedak, George. 1979. Cytogenetics of a barley x rye hybrid. Can. J.
Genet. Cytol. 21:543-548.
Rajhathy, T., J. W. Morrison, and S. Symko. 1963. Interspecific and
intergeneric hybrids in Hordeum. In Barley Genetics. I. Proc. 1st
Intl. Barley Genet. Symp., pp. 194-212.
BGN 10 toc
BGN Main Index