A Database for Triticeae and Avena
II. 24. A sodium azide induced multipistillate mutant in barley.
G. Prasad*, S.N. Mathur**, and D.K. Tripathi*. Department of Agricultural
Botany, S.D.J. Post Graduate College Chandeshwar, Azamgarh (U.P.) 276128;
and **Gorakhpur University, Gorakhpur, India. "R"
Sodium azide has been proved a highly efficient and effective mutagen
especially acting at S-phase (Nilan et al., 1973, 1975). A multipistillate
mutant was isolated from the M2 generation when 12 hr distilled water presoaked
seeds of barley cultivar DL 36 were treated with l0-3 M sodium
azide solution at pH3 for 2 hr. Observations showed that there was a group
of 5 pistils and it was bigger in size as compared to that of the mother
variety (Fig. 1a,b). Each ovary covered the next one partially and thus
their sizes were becoming successively smaller, the 5th ovary being the
smallest (Fig. 1c,d,e,f,g). However, only one of these ovarles could develop
into fruit. Seed setting in this mutant was very poor and more than 80
per cent of the spikelets were empty. Average pollen sterility was 10 per
cent (2.5 per cent in DL 365. The plant was vigorous, producing more tillers
and broader leaves as compared to DL 36.
Figure 1. Ovaries of multipistillate mutants.
a. multipistil, b. normal pistil (3-4 days after fertilization), c. (lst),
d. (2nd), e. (3rd), f. (4th), and g. (5th) pistils of a floret.
BGN 10 toc
BGN Main Index