BGN 10: A sodium azide induced multipistillate mutant in barley BARLEY GENETICS NEWSLETTER, VOL. 10, II. RESEARCH NOTES
Prasad et al., pp. 57-58

II. 24. A sodium azide induced multipistillate mutant in barley.

G. Prasad*, S.N. Mathur**, and D.K. Tripathi*. Department of Agricultural Botany, S.D.J. Post Graduate College Chandeshwar, Azamgarh (U.P.) 276128; and **Gorakhpur University, Gorakhpur, India. "R"

Sodium azide has been proved a highly efficient and effective mutagen especially acting at S-phase (Nilan et al., 1973, 1975). A multipistillate mutant was isolated from the M2 generation when 12 hr distilled water presoaked seeds of barley cultivar DL 36 were treated with l0-3 M sodium azide solution at pH3 for 2 hr. Observations showed that there was a group of 5 pistils and it was bigger in size as compared to that of the mother variety (Fig. 1a,b). Each ovary covered the next one partially and thus their sizes were becoming successively smaller, the 5th ovary being the smallest (Fig. 1c,d,e,f,g). However, only one of these ovarles could develop into fruit. Seed setting in this mutant was very poor and more than 80 per cent of the spikelets were empty. Average pollen sterility was 10 per cent (2.5 per cent in DL 365. The plant was vigorous, producing more tillers and broader leaves as compared to DL 36.

Figure 1. Ovaries of multipistillate mutants.  a. multipistil, b. normal pistil (3-4 days after fertilization), c. (lst), d. (2nd), e. (3rd), f. (4th), and g. (5th) pistils of a floret.

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