BGN 10: Haploid production in Hordeum spontaneum x H. bulbosum crosses BARLEY GENETICS NEWSLETTER, VOL. 10, II. RESEARCH NOTES
Simpson and Snape, pp. 66-67

II. 29. Haploid production in Hordeum spontaneum x H. bulbosum crosses.

E. Simpson and J.W. Snape, Plant Breeding Institute, Trumpington, Cambridge, England. "R"

Natural populations of Hordeum spontaneum undoubtedly contain genetical variation which could be usefully exploited in barley breeding programmes. One method of rapidly exploiting such variation might be to utilize the H. bulbosum technique to produce doubled haploid lines from H. spontaneum x H. vulgare hybrids. The present note reports a pilot study carried out to determine the extent of crossability between H. spontaneum and H. bulbosum and to examine, if crossing takes place, the frequencies of haploids produced from plants of natural populations of H. spontaneum from Israel. These form part of a collection obtained from Professor A. Dinoor of the Hebrew University, Jerusalem, which was partly sponsored by the Agricultural Research Council of Great Britain.

Three spring populations were examined and plants of these were crossed as female with the same genotype of diploid H. bulbosum. The overall frequencies obtained are shown in Table 1 and indicate that embryos were readily obtained from all genotypes. All were haploid H. spontaneum plants. Although the data were limited they also indicated that there might be variation between populations in their ability to produce embryos. However, no crossability problems were experienced suggesting that hybrids of H. spontaneum with H. vulgare should be capable of producing haploids when crossed with H. bulbosum.

Table 1. Embryos produced from crosses between H. spontaneum and diploid H. bulbosum.

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