BGN 12: Primary trisomlc analysis of the gene yst3 in barley BARLEY GENETICS NEWSLETTER, VOL. 12, II. RESEARCH NOTES
Tsuchiya and Shahla, pp. 31-32

II. 10. Primary trisomlc analysis of the gene yst3 in barley. (1)
Tsuchiya and Shahla, pp. 31-32

T. Tsuchiya and Azizeh Shahla, Department of Agronomy, Colorado State University, Fort Collins, Colorado 80523, U.S.A.

(1) Supported by USDA/SEA Competitive Research Grant 5901-0410-9-0334-0 and USDA/SEA Cooperative Research Grant 12-14-5001-265 and CSU Hatch project.

The gene yst3 (yellow streak 3) was found in the variety Lion (Singh and Tsuchiya, 1973). Prlmary trisomic analysis showed an association of this gene with chromosome 2 (Tsuchiya and Alanko, 1976). Telotrisomic analysis was conducted to associate this gene with the specific arm, 2L or 2S (Tsuchiya and Hang, 1974). The data obtained did not fit a trisomic ratio in either arm. Therefore, it was not possible to associate the yst3 gene with a specific arm of chromosome 2. To test the possible association of this gene with some other chromosome of barley, trisomic analysis was conducted with chromosome 7. Crosses were made between trisomic plants (Semierect) as female and mutant yst3 plants as the male parent. The segregation ratios were observed in the F2 generation (Table 1).

Table 1. F2 data for primary trisomic analysis of yst3 with Triplo 7 (Semierect).

The segregation ratio in F2 generation fits well to a 3:1 disomic ratio in the diploid portion and in the total population and two homozygous recessive plants were observed in the trisomic portion. Thus it seems to be safe to conclude that the gene yst3 is not associated with chromosome 7.

References:

Singh, R. J. and T. Tsuchiya. 1973. Allelism testing among three yellow streak
mutants. BGN 3:54-55.

Tsuchiya, T. and J. V. Alanko. 1976. Genetic studies of two mutations by means of primary trisomic analysis. BGN 6:84-85.

Tsuchiya, T. and A. Hang. 1979. Telotrisomic analysis of yst3 and i in barley. BGN 9:106-108.

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