GrainGenes

II. 3. Additional information on genetic analysis with acrotrisomic 1L^1S and four marker stocks.(1)

A. Shahla and T. Tsuchiya, Department of Agronomy, Colorado State University, Fort Collins, Colorado 80523 U.S.A.

(1) Supported by USDA Competitive Research Grant 82-CRCR-1-1020, USDA-CSU Cooperative Research Grant 58-9AHZ-2-265 and CSU Hatch Project.

Previous results from the genetic analysis of acrotrisomic 1L^1S and the triple marker stock f8, lk2, and n (Tsuchiya and Fujigaki, 1981; Shahla and Tsuchiya, 198 3 showed that f8 and lk2 are not located on the acrocentric chromosome 1L^1S, but the results for n character was not clear. In the present study all 3 marker genes were once again analyzed with acrotrisomic 1L^1S and the results are shown in Table 1.

Table 1. Segregation ratios in F2 generation in crosses of acrotrisomic 1L^1S with four marker stocks.

Gene n showed trisomic ratio with acrotrisomic 1L^1S and no homozygous trisomic was recovered for this mutant indicating that this gene is located in the intact segment on the long arm of acrocentric chromosome 1L^1S.

When a_c2 (albino seedling) was analyzed with this acrotrisomic (Table 1) segregation ratio did not fit to disomic (3:1) ratio, but the recovery of three homozygous recessive trisomics indicated that this gene is located on the missing segment of the short arm of acrocentric chromosome 1L^1S.

References:

Shahla, A. and T. Tsuchiya. 1983. Additional information of genetic analysis with acrotrisomic 1L^1S and triple marker stock f8, lk2, n. BGN 13:28-29.

Tsuchiya T. and J. Fujigaki. 1981. Genetic analysis with acrotrisomic 1L^1S. BGN 11:59-60.

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